caa a22 4500 467906858 CHVBK 20180406152836.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s00251-006-0129-y doi (NATIONALLICENCE)springer-10.1007/s00251-006-0129-y Gene structure and transcription of IRF-2 in the mandarin fish Siniperca chuatsi with the finding of alternative transcripts and microsatellite in the coding region [Elektronische Daten] [Baojian Sun, Mingxian Chang, Dali Chen, Pin Nie] The gene of interferon regulatory factor-2 (IRF-2) has been cloned from the mandarin fish (Siniperca chuatsi). The IRF-2 gene has 6,418 nucleotides (nt) and contains eight exons and seven introns, encoding two mRNAs. The two IRF-2 mRNAs each contained an open reading frame of 873 nt, which both translate into the same 291 amino acids but differed in their 5′ untranslated region: one mRNA was transcribed initially from the exon 1 bypassing exon 2, while the other was transcribed from the exon 2. The microsatellites (CA repeats) could be found in the carboxyl terminal region of mandarin fish IRF-2, which result in the truncated form molecules. The microsatellites' polymorphism was investigated, and eight alleles were found in 16 individuals. The microsatellites were also examined in IRF-2 of several freshwater perciform fishes. The transcription of the IRF-2 in different tissues with or without poly inosine-cytidine stimulation was analyzed by real-time PCR, and the constitutive transcription of both molecules could be detected in all the tissues examined. Springer-Verlag, 2006 IRF-2 nationallicence Gene organization nationallicence Microsatellite nationallicence Mandarin fish nationallicence Chinese perch nationallicence Siniperca chuatsi nationallicence Sun Baojian State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, Hubei Province, People's Republic of China aut Chang Mingxian State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, Hubei Province, People's Republic of China aut Chen Dali State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, Hubei Province, People's Republic of China aut Nie Pin State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, Hubei Province, People's Republic of China aut Immunogenetics Springer-Verlag 58/9(2006-09-01), 774-784 0093-7711 58:9<774 2006 58 251 https://doi.org/10.1007/s00251-006-0129-y text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0129-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sun Baojian State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, Hubei Province, People's Republic of China aut NATIONALLICENCE 700 1- Chang Mingxian State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, Hubei Province, People's Republic of China aut NATIONALLICENCE 700 1- Chen Dali State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, Hubei Province, People's Republic of China aut NATIONALLICENCE 700 1- Nie Pin State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, Hubei Province, People's Republic of China aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/9(2006-09-01), 774-784 0093-7711 58:9<774 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer