caa a22 4500 467906920 CHVBK 20180406152837.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s00251-006-0101-x doi (NATIONALLICENCE)springer-10.1007/s00251-006-0101-x Reference strand conformational analysis (RSCA) is a valuable tool in identifying MHC-DRB sequences in three species of Aotus monkeys [Elektronische Daten] [Juan Baquero, Santiago Miranda, Oscar Murillo, Heidy Mateus, Esperanza Trujillo, Carlos Suarez, Manuel Patarroyo, Carlos Parra-López] The Aotus monkey has been of great value in the pre-clinical study of malaria vaccine candidates. Several components of this primate's immune system have been studied and they display great similarity to their human counterparts. Cloning and sequencing studies have revealed extensive sequence polymorphisms in Aotus MHC-DRB with very high similarities to several human allelic lineages, grouping at least nine distinct MHC-DRB lineages. As the efficacy of peptide vaccines in this animal model may be strongly influenced by exon 2 MHC-DRB polymorphism, the availability of a reliable and rapid MHC-DRB typing method for three species of Aotus (Aotus nancymaae, Aotus vociferans and Aotus nigriceps) is necessary. Reference strand conformational analysis (RSCA) was used here for differentiating the distinctive Aotus MHC-DRB sequences' mobility using five fluorescently labelled references proved to be very useful for resolving closely related sequences, establishing the number of sequences transcribed in a particular monkey and their identity. The RSCA method's reliability in terms of identifying Aotus MHC-DRB sequences will facilitate evaluating individual responsiveness to vaccines and prompt studies associating susceptibility/resistance to infectious agents or auto-immune disease, for which Aotus monkeys may be considered to be an appropriate animal model. Springer-Verlag, 2006 Aotus monkeys nationallicence MHC-DRB nationallicence Reference strand conformational analysis (RSCA) nationallicence Fluorescently labelled reference (FLR) nationallicence Animal model nationallicence Baquero Juan Fundación Instituto de Inmunologia de Colombia (FIDIC), Carrera 50 Número 26-00, Bogotá, Colombia, South America aut Miranda Santiago Fundación Instituto de Inmunologia de Colombia (FIDIC), Carrera 50 Número 26-00, Bogotá, Colombia, South America aut Murillo Oscar Fundación Instituto de Inmunologia de Colombia (FIDIC), Carrera 50 Número 26-00, Bogotá, Colombia, South America aut Mateus Heidy Laboratorio de Biología Celular y Molecular, Facultad de Medicina, Universidad del Rosario, Calle 63 D Número 24-31, Bogotá, Colombia, South America aut Trujillo Esperanza Fundación Instituto de Inmunologia de Colombia (FIDIC), Carrera 50 Número 26-00, Bogotá, Colombia, South America aut Suarez Carlos Fundación Instituto de Inmunologia de Colombia (FIDIC), Carrera 50 Número 26-00, Bogotá, Colombia, South America aut Patarroyo Manuel Fundación Instituto de Inmunologia de Colombia (FIDIC), Carrera 50 Número 26-00, Bogotá, Colombia, South America aut Parra-López Carlos Fundación Instituto de Inmunologia de Colombia (FIDIC), Carrera 50 Número 26-00, Bogotá, Colombia, South America aut Immunogenetics Springer-Verlag 58/7(2006-07-01), 590-597 0093-7711 58:7<590 2006 58 251 https://doi.org/10.1007/s00251-006-0101-x text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0101-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baquero Juan Fundación Instituto de Inmunologia de Colombia (FIDIC), Carrera 50 Número 26-00, Bogotá, Colombia, South America aut NATIONALLICENCE 700 1- Miranda Santiago Fundación Instituto de Inmunologia de Colombia (FIDIC), Carrera 50 Número 26-00, Bogotá, Colombia, South America aut NATIONALLICENCE 700 1- Murillo Oscar Fundación Instituto de Inmunologia de Colombia (FIDIC), Carrera 50 Número 26-00, Bogotá, Colombia, South America aut NATIONALLICENCE 700 1- Mateus Heidy Laboratorio de Biología Celular y Molecular, Facultad de Medicina, Universidad del Rosario, Calle 63 D Número 24-31, Bogotá, Colombia, South America aut NATIONALLICENCE 700 1- Trujillo Esperanza Fundación Instituto de Inmunologia de Colombia (FIDIC), Carrera 50 Número 26-00, Bogotá, Colombia, South America aut NATIONALLICENCE 700 1- Suarez Carlos Fundación Instituto de Inmunologia de Colombia (FIDIC), Carrera 50 Número 26-00, Bogotá, Colombia, South America aut NATIONALLICENCE 700 1- Patarroyo Manuel Fundación Instituto de Inmunologia de Colombia (FIDIC), Carrera 50 Número 26-00, Bogotá, Colombia, South America aut NATIONALLICENCE 700 1- Parra-López Carlos Fundación Instituto de Inmunologia de Colombia (FIDIC), Carrera 50 Número 26-00, Bogotá, Colombia, South America aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/7(2006-07-01), 590-597 0093-7711 58:7<590 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer