caa a22 4500 467906955 CHVBK 20180406152837.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s00251-006-0133-2 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0133-2 A natural CCL5/RANTES variant antagonist for CCR1 and CCR3 [Elektronische Daten] [Corinne Capoulade-Métay, Ahidjo Ayouba, Anfumbom Kfutwah, Kavita Lole, Stéphane Pêtres, Yasmine Dudoit, Philippe Deterre, Elisabeth Menu, Françoise Barré-Sinoussi, Patrice Debré, Ioannis Theodorou] The N-terminal domain of the chemokine CCL5/regulated upon activation normal T cell expressed and secreted (RANTES) has been shown to be critical for its biological activity on leukocytes. Several N-terminus-modified CCL5/RANTES derivatives, such as N-Terminal truncated CCL5/RANTES, Met-RANTES, and amino-oxypentane (AOP)-RANTES exhibited antagonist or partial agonist functions when investigated on the properties of their receptors CCR1, CCR3, and CCR5. Studying 95 African samples from Cameroon, we found a naturally occurring variant of CCL5/RANTES containing a missense mutation located in the first amino acid of the secreted form (S24F). S24F binds CCR1, CCR3, and CCR5 and triggers receptor down-modulation comparable to CCL5/RANTES. Moreover, in CCR5 positive cells, S24F elicits cellular calcium mobilization equivalent to that obtained with CCL5/RANTES. By contrast, S24F does not provoke any response in CCR1 and CCR3 positive cells. As CCL5/RANTES is able to attract different subtypes of leukocytes into inflamed tissue and intervenes in a wide range of allergic and autoimmune diseases, the discovery of this natural N-terminus-modified CCL5/RANTES analogue exhibiting differential effects on CCL5/RANTES receptors, opens up additional perspectives for therapeutic intervention. Springer-Verlag, 2006 Chemokines nationallicence Chemokine receptors nationallicence Variant nationallicence Ca2+ mobilization nationallicence Capoulade-Métay Corinne INSERM U543, CHU Pitié-Salpêtrière, 91 Boulevard de l'Hôpital, 75013, Paris, France aut Ayouba Ahidjo Centre Pasteur du Cameroun, Yaoundé, Cameroon aut Kfutwah Anfumbom Centre Pasteur du Cameroun, Yaoundé, Cameroon aut Lole Kavita INSERM U543, CHU Pitié-Salpêtrière, 91 Boulevard de l'Hôpital, 75013, Paris, France aut Pêtres Stéphane Plateforme protéomique, Institut Pasteur, Paris, France aut Dudoit Yasmine INSERM U543, CHU Pitié-Salpêtrière, 91 Boulevard de l'Hôpital, 75013, Paris, France aut Deterre Philippe INSERM U543, CHU Pitié-Salpêtrière, 91 Boulevard de l'Hôpital, 75013, Paris, France aut Menu Elisabeth Unité de Régulation des Infections Rétrovirales, Institut Pasteur, Paris, France aut Barré-Sinoussi Françoise Unité de Régulation des Infections Rétrovirales, Institut Pasteur, Paris, France aut Debré Patrice INSERM U543, CHU Pitié-Salpêtrière, 91 Boulevard de l'Hôpital, 75013, Paris, France aut Theodorou Ioannis INSERM U543, CHU Pitié-Salpêtrière, 91 Boulevard de l'Hôpital, 75013, Paris, France aut Immunogenetics Springer-Verlag 58/7(2006-07-01), 533-541 0093-7711 58:7<533 2006 58 251 https://doi.org/10.1007/s00251-006-0133-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0133-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Capoulade-Métay Corinne INSERM U543, CHU Pitié-Salpêtrière, 91 Boulevard de l'Hôpital, 75013, Paris, France aut NATIONALLICENCE 700 1- Ayouba Ahidjo Centre Pasteur du Cameroun, Yaoundé, Cameroon aut NATIONALLICENCE 700 1- Kfutwah Anfumbom Centre Pasteur du Cameroun, Yaoundé, Cameroon aut NATIONALLICENCE 700 1- Lole Kavita INSERM U543, CHU Pitié-Salpêtrière, 91 Boulevard de l'Hôpital, 75013, Paris, France aut NATIONALLICENCE 700 1- Pêtres Stéphane Plateforme protéomique, Institut Pasteur, Paris, France aut NATIONALLICENCE 700 1- Dudoit Yasmine INSERM U543, CHU Pitié-Salpêtrière, 91 Boulevard de l'Hôpital, 75013, Paris, France aut NATIONALLICENCE 700 1- Deterre Philippe INSERM U543, CHU Pitié-Salpêtrière, 91 Boulevard de l'Hôpital, 75013, Paris, France aut NATIONALLICENCE 700 1- Menu Elisabeth Unité de Régulation des Infections Rétrovirales, Institut Pasteur, Paris, France aut NATIONALLICENCE 700 1- Barré-Sinoussi Françoise Unité de Régulation des Infections Rétrovirales, Institut Pasteur, Paris, France aut NATIONALLICENCE 700 1- Debré Patrice INSERM U543, CHU Pitié-Salpêtrière, 91 Boulevard de l'Hôpital, 75013, Paris, France aut NATIONALLICENCE 700 1- Theodorou Ioannis INSERM U543, CHU Pitié-Salpêtrière, 91 Boulevard de l'Hôpital, 75013, Paris, France aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/7(2006-07-01), 533-541 0093-7711 58:7<533 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer