caa a22 4500 467906971 CHVBK 20180406152837.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s00251-006-0131-4 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0131-4 Detailed characterization of the peptide binding specificity of five common Patr class I MHC molecules [Elektronische Daten] [John Sidney, Shinichi Asabe, Bjoern Peters, Kelly-Anne Purton, Josan Chung, Timothy Pencille, Robert Purcell, Christopher Walker, Francis Chisari, Alessandro Sette] The chimpanzee (Pan troglodytes) is an important model for studying the immune response to several human pathogens, but the study of correlates of immunity has been hindered by the fact that little is known about the epitope-binding specificity of chimpanzee (Patr) class I MHC. In the present study we have characterized the peptide binding specificity of several common Patr class I molecules. Using single amino acid substitution analogs and large peptide libraries, quantitative peptide binding motifs have been derived for Patr A*0101, A*0701, A*0901, B*0101, and B*2401. Each molecule was found to bind peptides using position 2 and the C terminus as main anchor contacts. On the other hand, each Patr molecule is associated with a unique binding specificity, and the range of specificities is similar to that seen amongst HLA alleles. A high degree of cross-reactivity was noted between Patr A*0701 and Patr A*0901, suggesting the existence of a Patr-specific supertype. Consistent with previous studies suggesting that some cross-reactivity may exist between HLA and Patr alleles, Patr A*0901 was found to have an appreciable degree of cross-reactivity with molecules of the HLA A24-supertype. Finally, utilizing motif scans and peptide binding and intracellular cytokine staining assays, 77 hepatitis B virus (HBV)-derived epitopes were identified in five chimpanzees that were recently convalescent from acute HBV infection. Because the Patr alleles studied herein were found to be very common in two different chimpanzee populations, the present data should facilitate the use of chimpanzees for immunological studies. Springer-Verlag, 2006 Major histocompatibility complex nationallicence Epitopes nationallicence Antigen presentation nationallicence Sidney John La Jolla Institute for Allergy and Immunology, 3030 Bunker Hill St., Ste. 326, 92109, San Diego, CA, USA aut Asabe Shinichi The Scripps Research Institute, 10550 North Torrey Pines Rd., 92037, La Jolla, CA, USA aut Peters Bjoern La Jolla Institute for Allergy and Immunology, 3030 Bunker Hill St., Ste. 326, 92109, San Diego, CA, USA aut Purton Kelly-Anne The Scripps Research Institute, 10550 North Torrey Pines Rd., 92037, La Jolla, CA, USA aut Chung Josan The Scripps Research Institute, 10550 North Torrey Pines Rd., 92037, La Jolla, CA, USA aut Pencille Timothy La Jolla Institute for Allergy and Immunology, 3030 Bunker Hill St., Ste. 326, 92109, San Diego, CA, USA aut Purcell Robert National Institute of Allergy and Infectious Diseases, 20892, Bethesda, MD, USA aut Walker Christopher The Ohio State University, and the Children's Research Institute, Columbus, OH, USA aut Chisari Francis The Scripps Research Institute, 10550 North Torrey Pines Rd., 92037, La Jolla, CA, USA aut Sette Alessandro La Jolla Institute for Allergy and Immunology, 3030 Bunker Hill St., Ste. 326, 92109, San Diego, CA, USA aut Immunogenetics Springer-Verlag 58/7(2006-07-01), 559-570 0093-7711 58:7<559 2006 58 251 https://doi.org/10.1007/s00251-006-0131-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0131-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sidney John La Jolla Institute for Allergy and Immunology, 3030 Bunker Hill St., Ste. 326, 92109, San Diego, CA, USA aut NATIONALLICENCE 700 1- Asabe Shinichi The Scripps Research Institute, 10550 North Torrey Pines Rd., 92037, La Jolla, CA, USA aut NATIONALLICENCE 700 1- Peters Bjoern La Jolla Institute for Allergy and Immunology, 3030 Bunker Hill St., Ste. 326, 92109, San Diego, CA, USA aut NATIONALLICENCE 700 1- Purton Kelly-Anne The Scripps Research Institute, 10550 North Torrey Pines Rd., 92037, La Jolla, CA, USA aut NATIONALLICENCE 700 1- Chung Josan The Scripps Research Institute, 10550 North Torrey Pines Rd., 92037, La Jolla, CA, USA aut NATIONALLICENCE 700 1- Pencille Timothy La Jolla Institute for Allergy and Immunology, 3030 Bunker Hill St., Ste. 326, 92109, San Diego, CA, USA aut NATIONALLICENCE 700 1- Purcell Robert National Institute of Allergy and Infectious Diseases, 20892, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Walker Christopher The Ohio State University, and the Children's Research Institute, Columbus, OH, USA aut NATIONALLICENCE 700 1- Chisari Francis The Scripps Research Institute, 10550 North Torrey Pines Rd., 92037, La Jolla, CA, USA aut NATIONALLICENCE 700 1- Sette Alessandro La Jolla Institute for Allergy and Immunology, 3030 Bunker Hill St., Ste. 326, 92109, San Diego, CA, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/7(2006-07-01), 559-570 0093-7711 58:7<559 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer