caa a22 4500 46790698X CHVBK 20180406152837.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s00251-006-0091-8 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0091-8 The chicken immunoregulatory receptor families SIRP, TREM, and CMRF35/CD300L [Elektronische Daten] [Birgit Viertlboeck, Ramona Schmitt, Thomas Göbel] Mammalian immunoregulatory families of genes encoding activating and inhibitory Ig-like receptor pairs have been located on distinct chromosomes. In chicken, a single Ig-like receptor family with many members had been described so far. By looking at sequence similarity and synteny conservations in the chicken genome, the signal-regulatory protein (SIRP), triggering receptor expressed on myeloid cells (TREM), and CMRF35/CD300L Ig-like gene families were identified on chromosomes 20, 26, and 3, respectively. Further analysis of the three corresponding genomic regions and partial bacterial artificial chromosome sequencing were used to identify more members and to realign several contigs. All putative genomic sequences were monitored by investigating existing expressed sequence tag and cloning cDNA. This approach yielded a single pair of activating and inhibitory SIRP, two inhibitory, and one activating TREM as well as one inhibitory CMRF35/CD300L with a potentially soluble variant and an additional member lacking categorizing motifs. The CMRF35/CD300L and TREM receptors were composed of one or two V-set Ig domains, whereas in SIRP, either a single Ig V domain was present or a combination of a V and C1 domains. Like in many Ig superfamily members, separate exons encode individual Ig domains. However, in two CMRF35/CD300L genes, the signal peptide and the distal Ig domain were encoded by a single exon. In conclusion, the mammalian diversity of immunoregulatory molecules is present the chicken suggesting an important role for TREM, SIRP, and CMRF35/CD300L in a functionally conserved network. Springer-Verlag, 2006 SIRP nationallicence TREM nationallicence CMRF35/CD300L nationallicence Phylogeny nationallicence Chicken nationallicence Viertlboeck Birgit Institute for Animal Physiology, University of Munich, Veterinärstr. 13, 80539, Munich, Germany aut Schmitt Ramona Institute for Animal Physiology, University of Munich, Veterinärstr. 13, 80539, Munich, Germany aut Göbel Thomas Institute for Animal Physiology, University of Munich, Veterinärstr. 13, 80539, Munich, Germany aut Immunogenetics Springer-Verlag 58/2-3(2006-04-01), 180-190 0093-7711 58:2-3<180 2006 58 251 https://doi.org/10.1007/s00251-006-0091-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0091-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Viertlboeck Birgit Institute for Animal Physiology, University of Munich, Veterinärstr. 13, 80539, Munich, Germany aut NATIONALLICENCE 700 1- Schmitt Ramona Institute for Animal Physiology, University of Munich, Veterinärstr. 13, 80539, Munich, Germany aut NATIONALLICENCE 700 1- Göbel Thomas Institute for Animal Physiology, University of Munich, Veterinärstr. 13, 80539, Munich, Germany aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/2-3(2006-04-01), 180-190 0093-7711 58:2-3<180 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer