caa a22 4500 467907056 CHVBK 20180406152837.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s00251-006-0092-7 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0092-7 Identification and characterization of porcine mannan-binding lectin A (pMBL-A), and determination of serum concentration heritability [Elektronische Daten] [Helle Juul-Madsen, Thomas Krogh-Meibom, Mark Henryon, Nades Palaniyar, Peter Heegaard, Stig Purup, Anthony Willis, Ida Tornøe, Klaus Ingvartsen, Søren Hansen, Uffe Holmskov] Mannan-binding lectin (MBL) is an innate immune collectin present in the serum of humans and many farm animals. This oligomeric pattern-recognition protein effectively binds to the glycoconjugate arrays present on the surfaces of microorganisms and activates the complement system to enhance pathogen killing and clearance. MBL deficiency is often associated with immunodeficiency in humans. Although two MBLs (MBL-A and MBL-C) have been characterized in various species, the identity of porcine MBL (pMBL) was not clearly defined. In this study, we purified an MBL from porcine serum by mannose affinity, ion exchange, and size exclusion chromatography and determined many of its characteristics. Based on the N-terminal sequence, multiple sequence alignment, and relative affinities to various carbohydrate ligands, we propose that the MBL purified in this study is pMBL-A. We have generated antibodies to this protein and established an immunoassay to quantify pMBL-A in serum. Using this assay, we found breed differences in pMBL-A concentration distributions and heritability estimates. In the Duroc breed (n=588), pMBL-A concentrations show a unimodal distribution with a mean of 9,125ng/ml. In contrast, the pMBL-A concentration distributions in the Landrace breed (n=533) show three distinct mean values: 301, 2,385, and 11,507ng/ml. Furthermore, heritability calculations based on an additive genetic variance model with no fixed effects indicate that serum pMBL-A concentration is highly heritable in the Landrace (h 2=0.8) but not in the Duroc breed (h 2=0.15). These genetic differences may be useful in selecting breeding pigs for improved disease resistance. Springer-Verlag, 2006 Acute phase reactants nationallicence Innate immunity nationallicence Mannan-binding lectin nationallicence Pigs nationallicence Infections nationallicence Juul-Madsen Helle Department of Animal Health, Welfare and Nutrition, Danish Institute of Agricultural Sciences, 8830, Tjele, Denmark aut Krogh-Meibom Thomas Department of Animal Health, Welfare and Nutrition, Danish Institute of Agricultural Sciences, 8830, Tjele, Denmark aut Henryon Mark Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, 8830, Tjele, Denmark aut Palaniyar Nades The Hospital for Sick Children Research Institute, Toronto, ON, Canada aut Heegaard Peter Department of Veterinary Diagnostics and Research, Danish Institute for Food and Veterinary Research, 1790, Copenhagen, Denmark aut Purup Stig Department of Animal Health, Welfare and Nutrition, Danish Institute of Agricultural Sciences, 8830, Tjele, Denmark aut Willis Anthony Department of Biochemistry, Medical Research Council Immunochemistry Unit, University of Oxford, Oxford, UK aut Tornøe Ida Medical Biotechnological Centre, University of Southern Denmark, 5000, Odense C, Denmark aut Ingvartsen Klaus Department of Animal Health, Welfare and Nutrition, Danish Institute of Agricultural Sciences, 8830, Tjele, Denmark aut Hansen Søren Medical Biotechnological Centre, University of Southern Denmark, 5000, Odense C, Denmark aut Holmskov Uffe Medical Biotechnological Centre, University of Southern Denmark, 5000, Odense C, Denmark aut Immunogenetics Springer-Verlag 58/2-3(2006-04-01), 129-137 0093-7711 58:2-3<129 2006 58 251 https://doi.org/10.1007/s00251-006-0092-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0092-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Juul-Madsen Helle Department of Animal Health, Welfare and Nutrition, Danish Institute of Agricultural Sciences, 8830, Tjele, Denmark aut NATIONALLICENCE 700 1- Krogh-Meibom Thomas Department of Animal Health, Welfare and Nutrition, Danish Institute of Agricultural Sciences, 8830, Tjele, Denmark aut NATIONALLICENCE 700 1- Henryon Mark Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, 8830, Tjele, Denmark aut NATIONALLICENCE 700 1- Palaniyar Nades The Hospital for Sick Children Research Institute, Toronto, ON, Canada aut NATIONALLICENCE 700 1- Heegaard Peter Department of Veterinary Diagnostics and Research, Danish Institute for Food and Veterinary Research, 1790, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Purup Stig Department of Animal Health, Welfare and Nutrition, Danish Institute of Agricultural Sciences, 8830, Tjele, Denmark aut NATIONALLICENCE 700 1- Willis Anthony Department of Biochemistry, Medical Research Council Immunochemistry Unit, University of Oxford, Oxford, UK aut NATIONALLICENCE 700 1- Tornøe Ida Medical Biotechnological Centre, University of Southern Denmark, 5000, Odense C, Denmark aut NATIONALLICENCE 700 1- Ingvartsen Klaus Department of Animal Health, Welfare and Nutrition, Danish Institute of Agricultural Sciences, 8830, Tjele, Denmark aut NATIONALLICENCE 700 1- Hansen Søren Medical Biotechnological Centre, University of Southern Denmark, 5000, Odense C, Denmark aut NATIONALLICENCE 700 1- Holmskov Uffe Medical Biotechnological Centre, University of Southern Denmark, 5000, Odense C, Denmark aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/2-3(2006-04-01), 129-137 0093-7711 58:2-3<129 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer