caa a22 4500 467907064 CHVBK 20180406152837.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s00251-006-0093-6 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0093-6 Gender-specific associations between MICA-STR and nasopharyngeal carcinoma in a southern Chinese Han population [Elektronische Daten] [Wei Tian, Xiao-min Zeng, Li-xin Li, He-kun Jin, Qi-zhi Luo, Fan Wang, Shi-shi Guo, Ya Cao] Previous studies have identified several HLA-B specificities that are associated with nasopharyngeal carcinoma (NPC) in populations of Chinese descent, in particular HLA-B35, -B38, -B46, and -B58. Perhaps except for HLA-B46, other associations cannot be simply accounted for by the linkage disequilibrium between HLA-A and B loci. The human major histocompatibility complex (MHC) class I chain-related gene A (MICA) maps 46kb centromeric to HLA-B and is highly polymorphic; it encodes a stress-inducible protein which functions as a ligand for the NKG2D/DAP10 complex to activate natural killer (NK) cells, γδ T cells, and CD8+ T cells. We postulated MICA gene as a susceptibility factor for nasopharyngeal carcinoma, an Epstein-Barr virus-associated malignancy. In this study, 218 unrelated patients newly diagnosed with NPC and 196 randomly selected healthy controls from southern China mainland were analyzed for the short tandem repeat polymorphism of exon 5 of MICA gene (MICA-STR) and MICA gene deletion, using fluorescent polymerase chain reaction-gene scanning (PCR/size-sequencing) and polymerase chain reaction-sequence-specific priming (PCR/SSP) technology. MICA*A9 was present at significantly increased frequency in the patient group (P C=0.0001002, OR=2.528, 95% CI=1.636-3.907), whereas the frequency of MICA*A5.1 was significantly decreased (P C=0.006, OR=0.594, 95% CI=0.437-0.806). Gender-based stratification revealed a significant increase of MICA*A9 frequency (P C=0.000072, OR=3.255, 95% CI=1.855-5.709) and a significant decrease of MICA*A5.1 frequency (P C=0.000737, OR=0.486, 95% CI=0.337-0.702) in male patients with NPC (N=166), compared with male normal controls (N=120). A significant interaction between MICA*A9 and gender was observed ( % MathType!Translator!2!1!AMS LaTeX.tdl!TeX -- AMS-LaTeX! % MathType!MTEF!2!1!+- % feaaeaart1ev0aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbbjxAHX % garmWu51MyVXgatuuDJXwAK1uy0HwmaeHbfv3ySLgzG0uy0Hgip5wz % aebbnrfifHhDYfgasaacH8qrps0lbbf9q8WrFfeuY-Hhbbf9v8qqaq % Fr0xc9pk0xbba9q8WqFfea0-yr0RYxir-Jbba9q8aq0-yq-He9q8qq % Q8frFve9Fve9Ff0dmeaabaqaciGacaGaaeqabaWaaeWaeaaakeaaca % qGybWaaSbaaSqaaiaadEfadaahaaadbeqaaiaaikdaaaaaleqaaaaa % !3A68! $${\text{X}}_{{W^{2} }} $$ =41.58, P=0.0001). Statistics also revealed heterogeneity of effects among MICA*A5.1/MICA*A9-bearing phenotypes and a dose-dependent effect of MICA*A5.1 and MICA*A9 on NPC risk in male subgroup. This constitutes the first demonstration of a gender-specific association between MICA-STR polymorphism and NPC, which could largely be attributable to the underlying gender-related mechanisms that modulate MICA gene expression. The results provide strong supporting evidence suggesting that MICA*A9 may be a genetic risk factor for NPC in male individuals in this population. The potential interaction between MICA and other non-HLA host factors and environmental exposures remains to be further studied. Springer-Verlag, 2006 Nasopharyngeal carcinoma nationallicence Human leukocyte antigen nationallicence MHC class-i chain-related gene A nationallicence Microsatellite DNA nationallicence Gender nationallicence Tian Wei Department of Immunology, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, People's Republic of China aut Zeng Xiao-min Department of Epidemiology and Statistics, Xiang-Ya School of Public Health, Central South University, 410078, Changsha, Hunan, People's Republic of China aut Li Li-xin Department of Immunology, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, People's Republic of China aut Jin He-kun Department of Radiotherapy, Oncology Hospital of Hunan Province, 410006, Changsha, Hunan, People's Republic of China aut Luo Qi-zhi Department of Immunology, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, People's Republic of China aut Wang Fan Department of Immunology, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, People's Republic of China aut Guo Shi-shi Department of Immunology, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, People's Republic of China aut Cao Ya Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, People's Republic of China aut Immunogenetics Springer-Verlag 58/2-3(2006-04-01), 113-121 0093-7711 58:2-3<113 2006 58 251 https://doi.org/10.1007/s00251-006-0093-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0093-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tian Wei Department of Immunology, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Zeng Xiao-min Department of Epidemiology and Statistics, Xiang-Ya School of Public Health, Central South University, 410078, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Li Li-xin Department of Immunology, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Jin He-kun Department of Radiotherapy, Oncology Hospital of Hunan Province, 410006, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Luo Qi-zhi Department of Immunology, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Wang Fan Department of Immunology, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Guo Shi-shi Department of Immunology, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Cao Ya Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, 410078, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/2-3(2006-04-01), 113-121 0093-7711 58:2-3<113 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer