caa a22 4500 467907072 CHVBK 20180406152837.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s00251-006-0096-3 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0096-3 Maternal transfer of complement components C3-1, C3-3, C3-4, C4, C5, C7, Bf, and Df to offspring in rainbow trout ( Oncorhynchus mykiss ) [Elektronische Daten] [Marie Løvoll, Terje Kilvik, Hani Boshra, Jarl Bøgwald, J. Sunyer, Roy Dalmo] Defense mechanisms in developing fish are poorly known but before maturation of lymphoid organs and immunocompetence, innate mechanisms are essential. The complement system represents a major part of innate immunity. Our main objective was to map the presence of complement components early in fish development. Rainbow trout eggs, embryos, and hatchlings were assayed for the onset and duration of C3-1, C3-3, C3-4, C4, C5, C7, factor B, and factor D transcription using real-time reverse transcription-polymerase chain reaction. In general, complement transcript levels increased steadily from day28 postfertilization to hatch, followed by a decrease during yolk-sac resorption. All the complement proteins studied were found in unfertilized eggs. There was no correlation between the transcript and protein levels throughout the study period. Complement proteins appeared in the liver, kidney, and intestine between day7 and 35 but not until day77 in the heart. This study is the first to address the ontogeny of several complement components and represents the first evidence that maternal transfer of complement components, other than C3, occurs in teleost fish. Springer-Verlag, 2006 Complement nationallicence Ontogeny nationallicence Rainbow trout nationallicence Løvoll Marie Department of Marine Biotechnology, Norwegian College of Fishery Science, University of Tromsø, 9037, Tromsø, Norway aut Kilvik Terje Department of Marine Biotechnology, Norwegian College of Fishery Science, University of Tromsø, 9037, Tromsø, Norway aut Boshra Hani Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, 19104, Philadelphia, PA, USA aut Bøgwald Jarl Department of Marine Biotechnology, Norwegian College of Fishery Science, University of Tromsø, 9037, Tromsø, Norway aut Sunyer J. Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, 19104, Philadelphia, PA, USA aut Dalmo Roy Department of Marine Biotechnology, Norwegian College of Fishery Science, University of Tromsø, 9037, Tromsø, Norway aut Immunogenetics Springer-Verlag 58/2-3(2006-04-01), 168-179 0093-7711 58:2-3<168 2006 58 251 https://doi.org/10.1007/s00251-006-0096-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0096-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Løvoll Marie Department of Marine Biotechnology, Norwegian College of Fishery Science, University of Tromsø, 9037, Tromsø, Norway aut NATIONALLICENCE 700 1- Kilvik Terje Department of Marine Biotechnology, Norwegian College of Fishery Science, University of Tromsø, 9037, Tromsø, Norway aut NATIONALLICENCE 700 1- Boshra Hani Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, 19104, Philadelphia, PA, USA aut NATIONALLICENCE 700 1- Bøgwald Jarl Department of Marine Biotechnology, Norwegian College of Fishery Science, University of Tromsø, 9037, Tromsø, Norway aut NATIONALLICENCE 700 1- Sunyer J. Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, 19104, Philadelphia, PA, USA aut NATIONALLICENCE 700 1- Dalmo Roy Department of Marine Biotechnology, Norwegian College of Fishery Science, University of Tromsø, 9037, Tromsø, Norway aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/2-3(2006-04-01), 168-179 0093-7711 58:2-3<168 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer