caa a22 4500 467907080 CHVBK 20180406152837.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s00251-005-0078-x doi (NATIONALLICENCE)springer-10.1007/s00251-005-0078-x NKG2D splice variants: a reexamination of adaptor molecule associations [Elektronische Daten] [Brian Rabinovich, Jennifer Li, Martin Wolfson, William Lawrence, Courtney Beers, Jan Chalupny, Rose Hurren, Brad Greenfield, Richard Miller, David Cosman] NKG2D is a homodimeric C-type lectin-related receptor expressed on natural killer (NK) cells and T cells. In mice, alternative deoxyribonucleic acid (DNA) splicing generates two isoforms of NKG2D that differ in the length of their cytoplasmic domains. Their ability to induce cellular activation is mediated via association with two membrane-bound, signaling adaptor molecules, DAP10 and DAP12. It has been reported that the long form of NKG2D associates exclusively with DAP10, whereas the short variant can interact with either adaptor. The short isoform was reported to be almost undetectable in naïve NK cells. Using two distinct cell types, we demonstrate that like the short isoform, the long variant of NKG2D also associates not only with DAP10 but also with DAP12. Using reporter cells (70Z/3), we demonstrate that DAP12 can compete equally with DAP10 for association with both variants of NKG2D when DAP10 and DAP12 are coexpressed. Cross-linking either isoform of NKG2D induces a calcium flux when associated exclusively with DAP10 or DAP12. Moreover, using quantitative polymerase chain reaction (PCR), we also show that the short isoform of NKG2D is expressed in naïve NK cells. Our data suggest that signaling via mouse NKG2D isoforms is more complex than originally presented. Springer-Verlag, 2006 Rodents nationallicence Natural killer cells nationallicence Activation receptors nationallicence Adaptor molecules nationallicence Alternative splicing nationallicence Rabinovich Brian MD Anderson Cancer Center, 7455 Fannin Street, 77654, Houston, TX, USA aut Li Jennifer Department of Medical Biophysics, University of Toronto, 610 University Avenue, M5G 2M9, Toronto, ON, Canada aut Wolfson Martin Amgen Washington, 1201 Amgen Court West, 98119, Seattle, WA, USA aut Lawrence William Amgen Washington, 1201 Amgen Court West, 98119, Seattle, WA, USA aut Beers Courtney Amgen Washington, 1201 Amgen Court West, 98119, Seattle, WA, USA aut Chalupny Jan Amgen Washington, 1201 Amgen Court West, 98119, Seattle, WA, USA aut Hurren Rose Department of Medical Biophysics, University of Toronto, 610 University Avenue, M5G 2M9, Toronto, ON, Canada aut Greenfield Brad Amgen Washington, 1201 Amgen Court West, 98119, Seattle, WA, USA aut Miller Richard Department of Medical Biophysics, University of Toronto, 610 University Avenue, M5G 2M9, Toronto, ON, Canada aut Cosman David Amgen Washington, 1201 Amgen Court West, 98119, Seattle, WA, USA aut Immunogenetics Springer-Verlag 58/2-3(2006-04-01), 81-88 0093-7711 58:2-3<81 2006 58 251 https://doi.org/10.1007/s00251-005-0078-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-005-0078-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rabinovich Brian MD Anderson Cancer Center, 7455 Fannin Street, 77654, Houston, TX, USA aut NATIONALLICENCE 700 1- Li Jennifer Department of Medical Biophysics, University of Toronto, 610 University Avenue, M5G 2M9, Toronto, ON, Canada aut NATIONALLICENCE 700 1- Wolfson Martin Amgen Washington, 1201 Amgen Court West, 98119, Seattle, WA, USA aut NATIONALLICENCE 700 1- Lawrence William Amgen Washington, 1201 Amgen Court West, 98119, Seattle, WA, USA aut NATIONALLICENCE 700 1- Beers Courtney Amgen Washington, 1201 Amgen Court West, 98119, Seattle, WA, USA aut NATIONALLICENCE 700 1- Chalupny Jan Amgen Washington, 1201 Amgen Court West, 98119, Seattle, WA, USA aut NATIONALLICENCE 700 1- Hurren Rose Department of Medical Biophysics, University of Toronto, 610 University Avenue, M5G 2M9, Toronto, ON, Canada aut NATIONALLICENCE 700 1- Greenfield Brad Amgen Washington, 1201 Amgen Court West, 98119, Seattle, WA, USA aut NATIONALLICENCE 700 1- Miller Richard Department of Medical Biophysics, University of Toronto, 610 University Avenue, M5G 2M9, Toronto, ON, Canada aut NATIONALLICENCE 700 1- Cosman David Amgen Washington, 1201 Amgen Court West, 98119, Seattle, WA, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/2-3(2006-04-01), 81-88 0093-7711 58:2-3<81 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer