caa a22 4500 467907099 CHVBK 20180406152837.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s00251-006-0085-6 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0085-6 Duplication, balancing selection and trans-species evolution explain the high levels of polymorphism of the DQA MHC class II gene in voles (Arvicolinae) [Elektronische Daten] [J. Bryja, M. Galan, N. Charbonnel, J. Cosson] Major histocompatibility complex (MHC) genes play important role in host-parasite interactions and parasites are crucial factors influencing the population dynamics of hosts. We described the structure and diversity of exon 2 of the MHC class II DQA gene in three species of voles (Arvicolinae) exhibiting regular multi-annual fluctuations of population density and analysed the processes leading to the observed MHC polymorphism. By using cloning-sequencing methodology and capillary electrophoresis-single strand conformation polymorphism, we described seven sequences in the water, eight in the common, and seven in the bank voles coming from an area of 70km2 around the Nozeroy canton in the Jura Mountains (Franche Comté, France). All exon 2 sequences translate to give unique amino acid sequences and positive selection was found to act very intensively on antigen binding sites. We documented the presence of recombination at vole DQA region but its importance in generating allelic polymorphism seems to be relatively limited. For the first time within rodents, we documented the duplication of the DQA gene in all three species with both copies being transcriptionally active. Phylogenetic analysis of allelic sequences revealed extensive trans-species polymorphism within the subfamily although no alleles were shared between species in our data set. We discuss possible role of parasites in forming the recent polymorphism pattern of the DQA locus in voles. Springer-Verlag, 2006 Muridae nationallicence Arvicola nationallicence Microtus nationallicence Clethrionomys nationallicence Allelic diversity nationallicence Bryja J. Centre de Biologie et Gestion des Populations (UMR 22), INRA, Campus International de Baillarguet, 34988 Montferrier sur Lez, CS 30016,, Cedex, France aut Galan M. Centre de Biologie et Gestion des Populations (UMR 22), INRA, Campus International de Baillarguet, 34988 Montferrier sur Lez, CS 30016,, Cedex, France aut Charbonnel N. Centre de Biologie et Gestion des Populations (UMR 22), INRA, Campus International de Baillarguet, 34988 Montferrier sur Lez, CS 30016,, Cedex, France aut Cosson J. Centre de Biologie et Gestion des Populations (UMR 22), INRA, Campus International de Baillarguet, 34988 Montferrier sur Lez, CS 30016,, Cedex, France aut Immunogenetics Springer-Verlag 58/2-3(2006-04-01), 191-202 0093-7711 58:2-3<191 2006 58 251 https://doi.org/10.1007/s00251-006-0085-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0085-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bryja J. Centre de Biologie et Gestion des Populations (UMR 22), INRA, Campus International de Baillarguet, 34988 Montferrier sur Lez, CS 30016,, Cedex, France aut NATIONALLICENCE 700 1- Galan M. Centre de Biologie et Gestion des Populations (UMR 22), INRA, Campus International de Baillarguet, 34988 Montferrier sur Lez, CS 30016,, Cedex, France aut NATIONALLICENCE 700 1- Charbonnel N. Centre de Biologie et Gestion des Populations (UMR 22), INRA, Campus International de Baillarguet, 34988 Montferrier sur Lez, CS 30016,, Cedex, France aut NATIONALLICENCE 700 1- Cosson J. Centre de Biologie et Gestion des Populations (UMR 22), INRA, Campus International de Baillarguet, 34988 Montferrier sur Lez, CS 30016,, Cedex, France aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/2-3(2006-04-01), 191-202 0093-7711 58:2-3<191 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer