caa a22 4500 467907102 CHVBK 20180406152837.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s00251-006-0090-9 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0090-9 Conservation and divergence of the Eμ3′ enhancer in the IGH locus of teleosts [Elektronische Daten] [Jun-ichi Hikima, Mara Lennard, Melanie Wilson, Norman Miller, L. Clem, Gregory Warr] The core region of the Eμ3′ transcriptional enhancer that drives the expression of the teleost IGH locus has been characterized functionally in two species, the catfish (Ictalurus punctatus) and the zebrafish (Danio rerio). These studies have suggested important differences: Whereas the catfish enhancer acts through an E-box and two octamer motifs, the zebrafish enhancer exerts its major effects through two E-box motifs alone. In this study, the function of the catfish enhancer was reexamined in a broader comparative context within the teleosts. Electrophoretic mobility shift assays of motifs from catfish, zebrafish, and Fugu were conducted to determine their ability to bind catfish E-protein and Oct transcription factors. Transient expression assays were conducted using a region of the catfish core enhancer that includes a newly described hybrid octamer/E-box motif. Sequences homologous to the Eμ3′ enhancer region from six teleosts were aligned to determine conserved regions ("phylogenetic footprinting”). These studies allowed the following conclusions to be drawn: (1) The important 3′E-box motif described in the zebrafish corresponds in the homologous region of the catfish enhancer to an Oct motif with a newly described negative regulatory function and (2) Comparison of the Eμ3′ enhancer sequences of six teleosts indicates that while a variety of octamer and E-box motifs are found in this region, strict evolutionary conservation of the important functional elements of the teleost Eμ3′ enhancer has not occurred. Springer-Verlag, 2006 Fish nationallicence IgH enhancer nationallicence Core region nationallicence E-protein nationallicence Oct transcription factor nationallicence Phylogenetic footprinting nationallicence Hikima Jun-ichi Marine Biomedicine and Environmental Sciences Center, Medical University of South Carolina, 29425, Charleston, SC, USA aut Lennard Mara Marine Biomedicine and Environmental Sciences Center, Medical University of South Carolina, 29425, Charleston, SC, USA aut Wilson Melanie Department of Microbiology, University of Mississippi Medical Center, 39216, Jackson, MS, USA aut Miller Norman Department of Microbiology, University of Mississippi Medical Center, 39216, Jackson, MS, USA aut Clem L. Department of Microbiology, University of Mississippi Medical Center, 39216, Jackson, MS, USA aut Warr Gregory Marine Biomedicine and Environmental Sciences Center, Medical University of South Carolina, 29425, Charleston, SC, USA aut Immunogenetics Springer-Verlag 58/2-3(2006-04-01), 226-234 0093-7711 58:2-3<226 2006 58 251 https://doi.org/10.1007/s00251-006-0090-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0090-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hikima Jun-ichi Marine Biomedicine and Environmental Sciences Center, Medical University of South Carolina, 29425, Charleston, SC, USA aut NATIONALLICENCE 700 1- Lennard Mara Marine Biomedicine and Environmental Sciences Center, Medical University of South Carolina, 29425, Charleston, SC, USA aut NATIONALLICENCE 700 1- Wilson Melanie Department of Microbiology, University of Mississippi Medical Center, 39216, Jackson, MS, USA aut NATIONALLICENCE 700 1- Miller Norman Department of Microbiology, University of Mississippi Medical Center, 39216, Jackson, MS, USA aut NATIONALLICENCE 700 1- Clem L. Department of Microbiology, University of Mississippi Medical Center, 39216, Jackson, MS, USA aut NATIONALLICENCE 700 1- Warr Gregory Marine Biomedicine and Environmental Sciences Center, Medical University of South Carolina, 29425, Charleston, SC, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/2-3(2006-04-01), 226-234 0093-7711 58:2-3<226 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer