caa a22 4500 467907110 CHVBK 20180406152837.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s00251-006-0086-5 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0086-5 Polymorphism of two very similar MHC class Ib loci in rainbow trout ( Oncorhynchus mykiss ) [Elektronische Daten] [Johannes Dijkstra, Ikunari Kiryu, Yasutoshi Yoshiura, Attila Kumánovics, Masakazu Kohara, Nobuhiro Hayashi, Mitsuru Ototake] As part of an ongoing elucidation of rainbow trout major histocompatibility complex (MHC) class I, the polymorphism of two MHC class Ib loci was analyzed. These loci, Onmy-UCA and Onmy-UDA, are situated head-to-tail and share more than 89% nucleotide identity in their open reading frames. They share 80% identity with some trout Ia alleles. The deduced amino acid sequences suggest that the UCA and UDA molecules are transported to endosomal compartments and may bind peptides in their binding groove. Our survey revealed seven UCA and eight UDA alleles. Similarity indices overlap when comparing within and between UCA and UDA alleles and some cross-locus motif variation is observed. In most trout both UCA and UDA transcripts were found. However, there probably is functional redundancy, because some trout lacked transcription of one of the two loci. Furthermore, for some UCA and UDA alleles, splicing deficiencies, early stop codons, and upstream start codons were found, which may interfere with efficient protein expression. The present study is the first extensive report on MHC class Ib polymorphism assigned to locus in ectotherm species. Springer-Verlag 2006, 2006 MHC nationallicence Polymorphism nationallicence Fish nationallicence Rainbow trout nationallicence Dijkstra Johannes Inland Station, Fisheries Research Agency, National Research Institute of Aquaculture, 519-0423, Tamaki, Mie, Japan aut Kiryu Ikunari Inland Station, Fisheries Research Agency, National Research Institute of Aquaculture, 519-0423, Tamaki, Mie, Japan aut Yoshiura Yasutoshi Inland Station, Fisheries Research Agency, National Research Institute of Aquaculture, 519-0423, Tamaki, Mie, Japan aut Kumánovics Attila Howard Hughes Medical Institute and Center for Immunology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, 75390-9050, Dallas, TX, USA aut Kohara Masakazu Nagano Prefectural Experimental Station of Fisheries, 399-7102, Akashina, Nagano, Japan aut Hayashi Nobuhiro Institute for Comprehensive Medical Science, Fujita Health University, 4701192, Toyoake, Aichi, Japan aut Ototake Mitsuru Inland Station, Fisheries Research Agency, National Research Institute of Aquaculture, 519-0423, Tamaki, Mie, Japan aut Immunogenetics Springer-Verlag 58/2-3(2006-04-01), 152-167 0093-7711 58:2-3<152 2006 58 251 https://doi.org/10.1007/s00251-006-0086-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0086-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dijkstra Johannes Inland Station, Fisheries Research Agency, National Research Institute of Aquaculture, 519-0423, Tamaki, Mie, Japan aut NATIONALLICENCE 700 1- Kiryu Ikunari Inland Station, Fisheries Research Agency, National Research Institute of Aquaculture, 519-0423, Tamaki, Mie, Japan aut NATIONALLICENCE 700 1- Yoshiura Yasutoshi Inland Station, Fisheries Research Agency, National Research Institute of Aquaculture, 519-0423, Tamaki, Mie, Japan aut NATIONALLICENCE 700 1- Kumánovics Attila Howard Hughes Medical Institute and Center for Immunology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, 75390-9050, Dallas, TX, USA aut NATIONALLICENCE 700 1- Kohara Masakazu Nagano Prefectural Experimental Station of Fisheries, 399-7102, Akashina, Nagano, Japan aut NATIONALLICENCE 700 1- Hayashi Nobuhiro Institute for Comprehensive Medical Science, Fujita Health University, 4701192, Toyoake, Aichi, Japan aut NATIONALLICENCE 700 1- Ototake Mitsuru Inland Station, Fisheries Research Agency, National Research Institute of Aquaculture, 519-0423, Tamaki, Mie, Japan aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/2-3(2006-04-01), 152-167 0093-7711 58:2-3<152 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer