caa a22 4500 467907129 CHVBK 20180406152837.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s00251-006-0097-2 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0097-2 Bovine T cell receptor gamma variable and constant genes: combinatorial usage by circulating γδ T cells [Elektronische Daten] [Carolyn Herzig, Seth Blumerman, Marie-Paule Lefranc, Cynthia Baldwin] Studies here describe expression and sequence of several new bovine T cell receptor gamma (TRG) genes to yield a total of 11 TRG variable (TRGV) genes (in eight subgroups) and six TRG constant (TRGC) genes. Publicly available genomic sequences were annotated to show their placement. Homologous TRG genes in cattle and sheep were assigned, using four accepted criteria. New genes described here include the bovine TRGC6, TRGV2, and TRGV4, homologues of ovine TRGC4, TRGV2, and TRGV4, respectively. The bovine Vγ7 and BTGV1 clones (previously TRGV4 and TRGV2, respectively) were reassigned to new subgroups TRGV7 and TRGV8, respectively, with approval by the IMGT Nomenclature Committee. Three TRGV subgroups (TRGV5, TRGV6, and TRGV8) were further designated as TRGV5-1 and TRGV5-2, TRGV6-1 and TRGV6-2, and TRGV8-1 and TRGV8-2 because each subgroup is comprised of two mapped genes. The complete sequence of bovine TRGC5 is also reported, for which a limited number of nucleotides was previously available, and shown to be most closely related to ovine TRGC5. Analysis of circulating γδ T cells revealed that rearrangement of TRGV genes with TRGC genes is largely dictated by their proximity within one of the six genomic V-J-C cassettes, with all TRG genes expressed by bovine peripheral blood γδ T cells. Cattle are useful models for γδ T cell biology because they have γδ T cells that respond to isopentenylpyrophosphate (IPP) antigens, while mice do not, and some bovine TRGV genes cluster closely with human genes. Springer-Verlag, 2006 γδ T cells nationallicence γδ T cell receptor genes nationallicence TRGC nationallicence TRGV nationallicence Ruminant nationallicence Herzig Carolyn Paige Laboratory, Department of Veterinary and Animal Sciences and Program in Molecular and Cellular Biology, University of Massachusetts, 01003, Amherst,, MA, USA aut Blumerman Seth Paige Laboratory, Department of Veterinary and Animal Sciences and Program in Molecular and Cellular Biology, University of Massachusetts, 01003, Amherst,, MA, USA aut Lefranc Marie-Paule Laboratoire d'ImmunoGénétique Moléculaire and Institut de Génétique Humaine, Université Montpellier II, 34396, Montpellier, France aut Baldwin Cynthia Paige Laboratory, Department of Veterinary and Animal Sciences and Program in Molecular and Cellular Biology, University of Massachusetts, 01003, Amherst,, MA, USA aut Immunogenetics Springer-Verlag 58/2-3(2006-04-01), 138-151 0093-7711 58:2-3<138 2006 58 251 https://doi.org/10.1007/s00251-006-0097-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0097-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Herzig Carolyn Paige Laboratory, Department of Veterinary and Animal Sciences and Program in Molecular and Cellular Biology, University of Massachusetts, 01003, Amherst,, MA, USA aut NATIONALLICENCE 700 1- Blumerman Seth Paige Laboratory, Department of Veterinary and Animal Sciences and Program in Molecular and Cellular Biology, University of Massachusetts, 01003, Amherst,, MA, USA aut NATIONALLICENCE 700 1- Lefranc Marie-Paule Laboratoire d'ImmunoGénétique Moléculaire and Institut de Génétique Humaine, Université Montpellier II, 34396, Montpellier, France aut NATIONALLICENCE 700 1- Baldwin Cynthia Paige Laboratory, Department of Veterinary and Animal Sciences and Program in Molecular and Cellular Biology, University of Massachusetts, 01003, Amherst,, MA, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/2-3(2006-04-01), 138-151 0093-7711 58:2-3<138 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer