caa a22 4500 467907137 CHVBK 20180406152837.0 cr unu---uuuuu 170328e20060401xx s 000 0 eng 10.1007/s00251-005-0069-y doi (NATIONALLICENCE)springer-10.1007/s00251-005-0069-y Paucity of class I MHC gene heterogeneity between individuals in the endangered Hawaiian monk seal population [Elektronische Daten] [Brian Aldridge, Lizabeth Bowen, Brett Smith, George Antonelis, Frances Gulland, Jeffrey Stott] The Hawaiian monk seal population has experienced precipitous declines in the last 50years. In this study, we provide evidence that individuals from remaining endangered population exhibit alarming uniformity in class I major histocompatibility (MHC) genes. The peripheral blood leukocyte-derived mRNA of six captive animals rescued from a stranding incident on the French frigate shoals in the Hawaiian archipelago was used to characterize genes in the monk seal class I MHC gene family, from which techniques for genotyping the broader population were designed using degenerate primers designed for the three major established human MHC class I loci (HLA-A, HLA-B, and HLA-C), and by sequencing multiple clones, six unique full-length classical MHC class I gene transcripts were identified among the six animals, three of which were only found in single individuals. Since The low degree of sequence variation between these transcripts and the similarity of genotype between individuals provided preliminary evidence for low class I MHC variability in the population. The sequence information from the class I transcripts from these six animals was used to design several primer sets for examining the extent of MHC variability in the remaining population using a combination of polymerase chain reaction and denaturing gradient gel electrophoresis (DGGE). Several DGGE assays, each one amplifying subtly different class I MHC gene combinations, were designed to compare exons encoding the highly polymorphic domains of the putative peptide-binding region of MHC class I. In combination, these assays failed to show interindividual variability at any of the class I MHC gene loci examined in either the six captive seals or in 80 free-ranging animals (∼6.7% of the estimated population) representing all six major subpopulations of Hawaiian monk seal. Springer-Verlag, 2006 Major histocompatibility complex nationallicence Hawaiian monk seal nationallicence Endangered species nationallicence Immunogenetics nationallicence Immunology nationallicence Aldridge Brian Veterinary Clinical Sciences, Royal Veterinary College, Hawkshead Lane, AL97TA, North Mymms, Herts, UK aut Bowen Lizabeth Laboratory for Marine Mammal Immunology, Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, 95616, Davis, CA, USA aut Smith Brett Laboratory for Marine Mammal Immunology, Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, 95616, Davis, CA, USA aut Antonelis George The Marine Mammal Center (TMMC), GGNRA, Marin Headlands, Sausalito, CA, USA aut Gulland Frances National Marine Fisheries Service, Honolulu, HI, USA aut Stott Jeffrey Laboratory for Marine Mammal Immunology, Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, 95616, Davis, CA, USA aut Immunogenetics Springer-Verlag 58/2-3(2006-04-01), 203-215 0093-7711 58:2-3<203 2006 58 251 https://doi.org/10.1007/s00251-005-0069-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-005-0069-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aldridge Brian Veterinary Clinical Sciences, Royal Veterinary College, Hawkshead Lane, AL97TA, North Mymms, Herts, UK aut NATIONALLICENCE 700 1- Bowen Lizabeth Laboratory for Marine Mammal Immunology, Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Smith Brett Laboratory for Marine Mammal Immunology, Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Antonelis George The Marine Mammal Center (TMMC), GGNRA, Marin Headlands, Sausalito, CA, USA aut NATIONALLICENCE 700 1- Gulland Frances National Marine Fisheries Service, Honolulu, HI, USA aut NATIONALLICENCE 700 1- Stott Jeffrey Laboratory for Marine Mammal Immunology, Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, 95616, Davis, CA, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/2-3(2006-04-01), 203-215 0093-7711 58:2-3<203 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer