caa a22 4500 467907153 CHVBK 20180406152837.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s00251-006-0155-9 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0155-9 Analysis of single nucleotide polymorphisms at 13 loci within the transforming growth factor-induced factor gene shows no association with high myopia in Japanese subjects [Elektronische Daten] [Yukiko Hasumi, Hidetoshi Inoko, Shuhei Mano, Masao Ota, Eiichi Okada, Jerzy Kulski, Ritsuko Nishizaki, Jeewon Mok, Akira Oka, Naoki Kumagai, Teruo Nishida, Shigeaki Ohno, Nobuhisa Mizuki] A previous study in China first indicated that the transforming growth factor-induced factor (TGIF) is a probable candidate gene for high myopia. The purpose of our study was to investigate whether there are significant associations between high myopia and single nucleotide polymorphism (SNP) variants in the TGIF gene of Japanese subjects. Genomic DNA was collected from 330 Japanese subjects with high myopia and at a level refractive error was less than −9.25Dsph and 330 randomized controls without high myopia. Thirteen SNPs were detected by polymerase chain reaction (PCR) and primer extension or by PCR and SNP-specific fluorogenic probes in all of the cases and controls. Thirteen SNPs were found within the TGIF genes of the cases and controls. Two of the SNPs were monomorphic and none of the 13 SNPs showed a significant result. The pairwise linkage disequilibrium (LD) mapping confirmed that these alleles have a comparatively strong LD index of >0.8 for D′ and >0.4 for r 2. We found no statistical association between any of the 13 SNPs located on the TGIF gene and high myopia in Japanese subjects. Based on our study using Japanese subjects and the previous studies of TGIF gene polymorphism in Chinese and northern European subjects with myopia, there is no convincing evidence to prove a connection between nucleotide sequence variations in TGIF and high myopia. Springer-Verlag, 2006 TGIF nationallicence Myopia nationallicence Japanese population nationallicence Hasumi Yukiko Department of Ophthalmology, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa, 236-0004, Yokohama, Japan aut Inoko Hidetoshi Department of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan aut Mano Shuhei Graduate School of Natural Sciences, Nagoya City University, Nagoya, Japan aut Ota Masao Department of Legal Medicine, Shinsyu University School of Medicine, Matsumoto, Nagano, Japan aut Okada Eiichi Okada Eye Clinic, Yokohama, Kanagawa, Japan aut Kulski Jerzy Department of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan aut Nishizaki Ritsuko Department of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan aut Mok Jeewon Department of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan aut Oka Akira Department of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan aut Kumagai Naoki Department of Ophthalmology, Yamaguchi University School of Medicine, Yamaguchi, Japan aut Nishida Teruo Department of Ophthalmology, Yamaguchi University School of Medicine, Yamaguchi, Japan aut Ohno Shigeaki Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Hokkaido, Japan aut Mizuki Nobuhisa Department of Ophthalmology, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa, 236-0004, Yokohama, Japan aut Immunogenetics Springer-Verlag 58/12(2006-12-01), 947-953 0093-7711 58:12<947 2006 58 251 https://doi.org/10.1007/s00251-006-0155-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0155-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hasumi Yukiko Department of Ophthalmology, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa, 236-0004, Yokohama, Japan aut NATIONALLICENCE 700 1- Inoko Hidetoshi Department of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan aut NATIONALLICENCE 700 1- Mano Shuhei Graduate School of Natural Sciences, Nagoya City University, Nagoya, Japan aut NATIONALLICENCE 700 1- Ota Masao Department of Legal Medicine, Shinsyu University School of Medicine, Matsumoto, Nagano, Japan aut NATIONALLICENCE 700 1- Okada Eiichi Okada Eye Clinic, Yokohama, Kanagawa, Japan aut NATIONALLICENCE 700 1- Kulski Jerzy Department of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan aut NATIONALLICENCE 700 1- Nishizaki Ritsuko Department of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan aut NATIONALLICENCE 700 1- Mok Jeewon Department of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan aut NATIONALLICENCE 700 1- Oka Akira Department of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan aut NATIONALLICENCE 700 1- Kumagai Naoki Department of Ophthalmology, Yamaguchi University School of Medicine, Yamaguchi, Japan aut NATIONALLICENCE 700 1- Nishida Teruo Department of Ophthalmology, Yamaguchi University School of Medicine, Yamaguchi, Japan aut NATIONALLICENCE 700 1- Ohno Shigeaki Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Hokkaido, Japan aut NATIONALLICENCE 700 1- Mizuki Nobuhisa Department of Ophthalmology, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa, 236-0004, Yokohama, Japan aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/12(2006-12-01), 947-953 0093-7711 58:12<947 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer