caa a22 4500 46790717X CHVBK 20180406152837.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s00251-006-0164-8 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0164-8 MHC class I allele frequencies in pigtail macaques of diverse origin [Elektronische Daten] [Bridget Pratt, David O'Connor, Bernard Lafont, Joseph Mankowski, Caroline Fernandez, Retno Triastuti, Andrew Brooks, Stephen Kent, Miranda Smith] Pigtail macaques (Macaca nemestrina) are an increasingly common primate model for the study of human AIDS. Major Histocompatibility complex (MHC) class I-restricted CD8+ T cell responses are a critical part of the adaptive immune response to HIV-1 in humans and simian immunodeficiency virus (SIV) in macaques; however, MHC class I alleles have not yet been comprehensively characterized in pigtail macaques. The frequencies of ten previously defined alleles (four Mane-A and six Mane-B) were investigated in detail in 109 pigtail macaques using reference strand-mediated conformational analysis (RSCA). The macaques were derived from three separate breeding colonies in the USA, Indonesia and Australia, and allele frequencies were analysed within and between these groups. Mane-A*10, an allele that restricts the immunodominant SIV Gag epitope KP9, was the most common allele, present in 32.1% of the animals overall, with similar frequencies across the three cohorts. Additionally, RSCA identified a new allele (Mane-A*17) common to three Indonesian pigtail macaques responding to the same Gag CD8+ T cell epitope. This broad characterization of common MHC class I alleles in more than 100 pigtail macaques further develops this animal model for the study of virus-specific CD8+ T cell responses. Springer-Verlag, 2006 Pigtail macaques nationallicence MHC class I nationallicence Reference strand-mediated conformational analysis (RSCA) nationallicence CD8+ T cell nationallicence Pratt Bridget Department of Microbiology and Immunology, University of Melbourne, 3010, Melbourne, Victoria, Australia aut O'Connor David Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI, USA aut Lafont Bernard Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA aut Mankowski Joseph Department of Molecular and Comparative Pathobiology, Johns Hopkins University, Baltimore, MD, USA aut Fernandez Caroline Department of Microbiology and Immunology, University of Melbourne, 3010, Melbourne, Victoria, Australia aut Triastuti Retno Primate Research Center, Bogor Agricultural University, Bogor, Indonesia aut Brooks Andrew Department of Microbiology and Immunology, University of Melbourne, 3010, Melbourne, Victoria, Australia aut Kent Stephen Department of Microbiology and Immunology, University of Melbourne, 3010, Melbourne, Victoria, Australia aut Smith Miranda Department of Microbiology and Immunology, University of Melbourne, 3010, Melbourne, Victoria, Australia aut Immunogenetics Springer-Verlag 58/12(2006-12-01), 995-1001 0093-7711 58:12<995 2006 58 251 https://doi.org/10.1007/s00251-006-0164-8 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0164-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pratt Bridget Department of Microbiology and Immunology, University of Melbourne, 3010, Melbourne, Victoria, Australia aut NATIONALLICENCE 700 1- O'Connor David Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI, USA aut NATIONALLICENCE 700 1- Lafont Bernard Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Mankowski Joseph Department of Molecular and Comparative Pathobiology, Johns Hopkins University, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Fernandez Caroline Department of Microbiology and Immunology, University of Melbourne, 3010, Melbourne, Victoria, Australia aut NATIONALLICENCE 700 1- Triastuti Retno Primate Research Center, Bogor Agricultural University, Bogor, Indonesia aut NATIONALLICENCE 700 1- Brooks Andrew Department of Microbiology and Immunology, University of Melbourne, 3010, Melbourne, Victoria, Australia aut NATIONALLICENCE 700 1- Kent Stephen Department of Microbiology and Immunology, University of Melbourne, 3010, Melbourne, Victoria, Australia aut NATIONALLICENCE 700 1- Smith Miranda Department of Microbiology and Immunology, University of Melbourne, 3010, Melbourne, Victoria, Australia aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/12(2006-12-01), 995-1001 0093-7711 58:12<995 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer