caa a22 4500 46790720X CHVBK 20180406152838.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s00251-006-0160-z doi (NATIONALLICENCE)springer-10.1007/s00251-006-0160-z Single-nucleotide polymorphisms in porcine mannan-binding lectin A [Elektronische Daten] [Brandon Lillie, Natalie Keirstead, E. Squires, M. Hayes] The MBL1 and MBL2 genes encode mannan-binding lectins (MBL) A and C, respectively, that are collagenous lectins (collectins) produced mainly by the liver. Several single-nucleotide polymorphisms (SNPs) in the human MBL2 gene are responsible for various innate immune dysfunctions due to abnormal structure or expression of human MBL-C. The MBL1 gene encodes MBL-A, which has bacteria-binding properties in pigs and rodents but is mutated to a pseudogene in humans and chimpanzees. In these studies, we surveyed both porcine MBL genes for SNPs that might impair disease resistance. Single-strand conformational polymorphism (SSCP) analysis of MBL cDNAs from porcine liver revealed three SNPs within the coding region of MBL1 in various breeds of pigs. One nonsynonymous SNP that substituted cysteine for glycine in the collagen-like domain of pig MBL-A was found by a multiplex PCR test in all European pig breeds examined, with allele frequencies ranging from 1.4 to 46.4%. No SNPs were identified in the coding region of porcine MBL2 but the expression of MBL-C in the liver was widely variable in comparison to the expression of MBL-A, GAPDH, PigMAP, and haptoglobin. These results indicate that some pigs have a miscoding defect in MBL-A and a possible expression defect in MBL-C, which are analogous to coding and promoter polymorphisms that affect human MBL-C. Springer-Verlag, 2006 Mannan (mannose)-binding lectin nationallicence Collectin nationallicence Innate immunity nationallicence Polymorphism nationallicence Pig nationallicence Lillie Brandon Department of Pathobiology, Ontario Veterinary College, University of Guelph, N1G 2W1, Guelph, ON, Canada aut Keirstead Natalie Department of Pathobiology, Ontario Veterinary College, University of Guelph, N1G 2W1, Guelph, ON, Canada aut Squires E. Department of Animal and Poultry Science, University of Guelph, N1G 2W1, Guelph, ON, Canada aut Hayes M. Department of Pathobiology, Ontario Veterinary College, University of Guelph, N1G 2W1, Guelph, ON, Canada aut Immunogenetics Springer-Verlag 58/12(2006-12-01), 983-993 0093-7711 58:12<983 2006 58 251 https://doi.org/10.1007/s00251-006-0160-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0160-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lillie Brandon Department of Pathobiology, Ontario Veterinary College, University of Guelph, N1G 2W1, Guelph, ON, Canada aut NATIONALLICENCE 700 1- Keirstead Natalie Department of Pathobiology, Ontario Veterinary College, University of Guelph, N1G 2W1, Guelph, ON, Canada aut NATIONALLICENCE 700 1- Squires E. Department of Animal and Poultry Science, University of Guelph, N1G 2W1, Guelph, ON, Canada aut NATIONALLICENCE 700 1- Hayes M. Department of Pathobiology, Ontario Veterinary College, University of Guelph, N1G 2W1, Guelph, ON, Canada aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/12(2006-12-01), 983-993 0093-7711 58:12<983 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer