caa a22 4500 467907226 CHVBK 20180406152838.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s00251-006-0139-9 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0139-9 Structure of the catfish IGH locus: analysis of the region including the single functional IGHM gene [Elektronische Daten] [E. Bengtén, S. Quiniou, J. Hikima, G. Waldbieser, G. Warr, N. Miller, M. Wilson] The catfish IGH locus is large (∼1Mb) and complex, having undergone multiple internal duplications and transpositions. To define the structure of the locus that contains the single expressed IGHM gene, two overlapping bacterial-artificial-chromosome (BAC) clones spanning the most 3′ end of the channel catfish immunoglobulin heavy (IGH) chain locus have been completely sequenced. The analyses created a contig of 257,153bp containing 55VH, 6D, 12JH genes and the IGH constant region genes encoding the functional secreted and membrane forms of IgM and the membrane form of IgD. This analysis revealed three major features. First, no C-region genes were found aside from the previously described IGHM1 and IGHD1, with the latter gene being the most 3′ C-region gene of the catfish IGH locus. There was no evidence in the region sequenced for genes that could encode an Ig class similar to the IgZ/IgT described in zebrafish, trout and pufferfish. Second, there are a high number of VH pseudogenes, 28 out of 55 (51%). In contrast, the entire zebrafish IGH locus has 40 functional VH genes and eight pseudogenes (17%). Third, an internal duplication of a 52.4-kb block of VH genes has occurred. These observations suggest that the IGH locus of teleost fish varies significantly from species to species in the diversity of C-region genes as well as the numbers of genes encoding V regions. Springer-Verlag, 2006 Immunoglobulin heavy chain locus nationallicence Catfish nationallicence Bacterial artificial chromosome nationallicence Bengtén E. Department of Microbiology, University of Mississippi Medical Center, 39216, Jackson, MS, USA aut Quiniou S. Catfish Genetics Research Unit, ARS, USDA, 141 Experimental Station Road, 38701, Stoneville, MS, USA aut Hikima J. Marine Biomedicine and Environmental Sciences Center and Department of Biochemistry and Molecular Biology, Medical University of South Carolina, 29425, Charleston, SC, USA aut Waldbieser G. Catfish Genetics Research Unit, ARS, USDA, 141 Experimental Station Road, 38701, Stoneville, MS, USA aut Warr G. Marine Biomedicine and Environmental Sciences Center and Department of Biochemistry and Molecular Biology, Medical University of South Carolina, 29425, Charleston, SC, USA aut Miller N. Department of Microbiology, University of Mississippi Medical Center, 39216, Jackson, MS, USA aut Wilson M. Department of Microbiology, University of Mississippi Medical Center, 39216, Jackson, MS, USA aut Immunogenetics Springer-Verlag 58/10(2006-10-01), 831-844 0093-7711 58:10<831 2006 58 251 https://doi.org/10.1007/s00251-006-0139-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0139-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bengtén E. Department of Microbiology, University of Mississippi Medical Center, 39216, Jackson, MS, USA aut NATIONALLICENCE 700 1- Quiniou S. Catfish Genetics Research Unit, ARS, USDA, 141 Experimental Station Road, 38701, Stoneville, MS, USA aut NATIONALLICENCE 700 1- Hikima J. Marine Biomedicine and Environmental Sciences Center and Department of Biochemistry and Molecular Biology, Medical University of South Carolina, 29425, Charleston, SC, USA aut NATIONALLICENCE 700 1- Waldbieser G. Catfish Genetics Research Unit, ARS, USDA, 141 Experimental Station Road, 38701, Stoneville, MS, USA aut NATIONALLICENCE 700 1- Warr G. Marine Biomedicine and Environmental Sciences Center and Department of Biochemistry and Molecular Biology, Medical University of South Carolina, 29425, Charleston, SC, USA aut NATIONALLICENCE 700 1- Miller N. Department of Microbiology, University of Mississippi Medical Center, 39216, Jackson, MS, USA aut NATIONALLICENCE 700 1- Wilson M. Department of Microbiology, University of Mississippi Medical Center, 39216, Jackson, MS, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/10(2006-10-01), 831-844 0093-7711 58:10<831 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer