caa a22 4500 467907234 CHVBK 20180406152838.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s00251-006-0156-8 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0156-8 Molecular study of Mhc-DRB in wild chacma baboons reveals high variability and evidence for trans-species inheritance [Elektronische Daten] [Elise Huchard, Guy Cowlishaw, Michel Raymond, Mylene Weill, Leslie Knapp] The MHC class II genes of many primate species were investigated extensively in recent years. However, while Mhc-DRB genes were studied in Old World monkeys such as rhesus macaques, the Mhc-DRB of baboons was only studied in a limited way. Because of their close anatomical and physiological relationship to humans, baboons are often used as models for reproduction and transplantation research. Baboons are also studied as a model species in behavioural ecology. Thus, identification of MHC genes would provide a foundation for studies of Mhc, biology and behaviour. Here, we describe the use of PCR, cloning, denaturing gradient gel electrophoresis (DGGE) and sequencing to identify Mhc-DRB sequences in wild chacma baboons (Papio ursinus). We amplified the highly variable second exon of baboon Mhc-DRB sequences using generic DRB primers. To validate and optimize the DGGE protocol, four DNA samples were initially studied using cloning and sequencing. Clones were screened using a novel RFLP approach to increase the number of clones identified for each individual. Results from cloning and sequencing were used to optimise DGGE conditions for Mhc-DRB genotyping of the remaining study subjects. Using these techniques, we identified 16 Paur-DRB sequences from 30 chacma baboons. On the basis of phylogenetic tree analyses, representatives of the Mhc-DRB1 and Mhc-DRB5 loci, and 13 different DRB lineages were identified. Evidence for trans-species inheritance of some Mhc-DRB sequences comes from high identity between the new Paur-DRB sequences and sequences from Papio cynocephalus, Macaca mulatta and possibly Galago moholi. Springer-Verlag, 2006 Old world monkey nationallicence Chacma baboon nationallicence Mhc-DRB nationallicence Denaturing gradient gel electrophoresis nationallicence Cloning nationallicence Sequencing nationallicence RFLP nationallicence Huchard Elise Equipe Génétique et Environnement, Institut des Sciences de l' Evolution (CNRS-UMR 5554), Université Montpellier II (CC 65), Place Eugène Bataillon, 34095, Montpellier, France aut Cowlishaw Guy Institute of Zoology, Zoological Society of London, Regent's Park, NW1 4RY, London, UK aut Raymond Michel Equipe Génétique et Environnement, Institut des Sciences de l' Evolution (CNRS-UMR 5554), Université Montpellier II (CC 65), Place Eugène Bataillon, 34095, Montpellier, France aut Weill Mylene Equipe Génétique et Environnement, Institut des Sciences de l' Evolution (CNRS-UMR 5554), Université Montpellier II (CC 65), Place Eugène Bataillon, 34095, Montpellier, France aut Knapp Leslie Department of Biological Anthropology, University of Cambridge, Downing Street, CB2 3DZ, Cambridge, UK aut Immunogenetics Springer-Verlag 58/10(2006-10-01), 805-816 0093-7711 58:10<805 2006 58 251 https://doi.org/10.1007/s00251-006-0156-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0156-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huchard Elise Equipe Génétique et Environnement, Institut des Sciences de l' Evolution (CNRS-UMR 5554), Université Montpellier II (CC 65), Place Eugène Bataillon, 34095, Montpellier, France aut NATIONALLICENCE 700 1- Cowlishaw Guy Institute of Zoology, Zoological Society of London, Regent's Park, NW1 4RY, London, UK aut NATIONALLICENCE 700 1- Raymond Michel Equipe Génétique et Environnement, Institut des Sciences de l' Evolution (CNRS-UMR 5554), Université Montpellier II (CC 65), Place Eugène Bataillon, 34095, Montpellier, France aut NATIONALLICENCE 700 1- Weill Mylene Equipe Génétique et Environnement, Institut des Sciences de l' Evolution (CNRS-UMR 5554), Université Montpellier II (CC 65), Place Eugène Bataillon, 34095, Montpellier, France aut NATIONALLICENCE 700 1- Knapp Leslie Department of Biological Anthropology, University of Cambridge, Downing Street, CB2 3DZ, Cambridge, UK aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/10(2006-10-01), 805-816 0093-7711 58:10<805 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer