caa a22 4500 467907250 CHVBK 20180406152838.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s00251-006-0143-0 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0143-0 Molecular cloning and expression of the porcine high-affinity immunoglobulin G Fc receptor (FcγRI) [Elektronische Daten] [Gaiping Zhang, Songlin Qiao, Qingmei Li, Xuannian Wang, Yanhua Duan, Li Wang, Zhijun Xiao, Chun Xia] Receptors for the Fc region (FcγRs) of immunoglobulin G (IgG) play a crucial role in the immune system and host protection against infection. In this study, we describe the cloning, sequencing, and expression of the high-affinity IgG receptor from pig. By screening a translated Expressed Sequence Tags database with the human FcγRI (CD64) protein sequence, we identified a putative porcine homologue. Subsequent polymerase chain reaction amplification confirmed that the identified full-length cDNA was expressed in porcine cells. Rosetting analysis shows that COS-7 cells transfected with a plasmid containing the cloned cDNA were able to bind chicken erythrocytes sensitized with porcine IgG. Scatchard analysis indicated that monomeric IgG bound to transiently transfected cells with an affinity of approximately 4×107M−1. The porcine FcγRI cDNA is 1,038 nucleotides long and is predicted to encode a 346-amino-acid transmembrane glycoprotein composed of three Ig-like domains, a transmembrane region, and a short cytoplasmic tail. The overall identity of the porcine FcγRI to its human and mouse counterparts at the level of the amino acid sequence was 75% and 57%, respectively. Identification of porcine FcγRI will aid in the understanding of the molecular basis of the porcine immune system and further studies of the receptor function. Springer-Verlag, 2006 Cloning nationallicence Expression nationallicence Pig nationallicence Receptor nationallicence Zhang Gaiping College of Animal and Veterinary Sciences, Henan Institute of Science and Technology, 453003, Xinxiang, China aut Qiao Songlin College of Veterinary Medicine, Henan Agricultural University, 450002, Zhengzhou, China aut Li Qingmei Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, 450002, Zhenzhou, China aut Wang Xuannian College of Animal and Veterinary Sciences, Henan Institute of Science and Technology, 453003, Xinxiang, China aut Duan Yanhua College of Veterinary Medicine, Henan Agricultural University, 450002, Zhengzhou, China aut Wang Li College of Veterinary Medicine, Henan Agricultural University, 450002, Zhengzhou, China aut Xiao Zhijun Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, 450002, Zhenzhou, China aut Xia Chun College of Veterinary Medicine, China Agricultural University, 100094, Beijing, China aut Immunogenetics Springer-Verlag 58/10(2006-10-01), 845-849 0093-7711 58:10<845 2006 58 251 https://doi.org/10.1007/s00251-006-0143-0 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0143-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Gaiping College of Animal and Veterinary Sciences, Henan Institute of Science and Technology, 453003, Xinxiang, China aut NATIONALLICENCE 700 1- Qiao Songlin College of Veterinary Medicine, Henan Agricultural University, 450002, Zhengzhou, China aut NATIONALLICENCE 700 1- Li Qingmei Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, 450002, Zhenzhou, China aut NATIONALLICENCE 700 1- Wang Xuannian College of Animal and Veterinary Sciences, Henan Institute of Science and Technology, 453003, Xinxiang, China aut NATIONALLICENCE 700 1- Duan Yanhua College of Veterinary Medicine, Henan Agricultural University, 450002, Zhengzhou, China aut NATIONALLICENCE 700 1- Wang Li College of Veterinary Medicine, Henan Agricultural University, 450002, Zhengzhou, China aut NATIONALLICENCE 700 1- Xiao Zhijun Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, 450002, Zhenzhou, China aut NATIONALLICENCE 700 1- Xia Chun College of Veterinary Medicine, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/10(2006-10-01), 845-849 0093-7711 58:10<845 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer