caa a22 4500 467907285 CHVBK 20180406152838.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s00251-006-0146-x doi (NATIONALLICENCE)springer-10.1007/s00251-006-0146-x IL-1 Receptor antagonist as a positional candidate gene in a murine model of allergic asthma [Elektronische Daten] [Ravisankar Ramadas, Xingnan Li, Dennis Shubitowski, Sridhar Samineni, Marsha Wills-Karp, Susan Ewart] Interleukin-1 receptor antagonist (IL-1ra) is an inhibitor of the proinflammatory IL-1. The IL-1ra gene (Il1rn) maps near the allergen-induced bronchial hyper-responsiveness-1 locus, Abhr1, which we previously mapped to murine chromosome 2 using A/J (asthma susceptible) and C3H/HeJ (asthma resistant) mice. We evaluated the role of Il1rn in our mouse model by comparing its genomic sequence between A/J and C3H/HeJ mice as well as assessing strain-specific RNA and protein production in response to allergen. We identified no functional sequence variations in the Il1rn gene between A/J and C3H/HeJ mice. Il1rn mRNA and protein were induced by ovalbumin (OVA) exposure in both strains, but to a greater extent in A/J mice at the earlier time points. We examined other IL-1 family members (Il1a, Il1b, Il1f9, and Il1r2) and found OVA-induced expression increases at 6h, yet only Il1b and Il1f9 had strain-specific differences. Of these, only Il1f9 is located within Abhr1, and we found several non-coding polymorphisms in the Il1f9 gene between A/J and C3H/HeJ mice. Our results exclude Il1rn as the gene for Abhr1 and indicate that Il1f9 warrants further investigation based on genetic and expression differences observed in our mouse model of allergic asthma. Springer-Verlag, 2006 IL-1 receptor antagonist nationallicence Asthma nationallicence Genetics nationallicence Airway inflammation nationallicence Mouse nationallicence Ramadas Ravisankar Comparative Medicine and Integrative Biology Program, College of Veterinary Medicine, Michigan State University, 48824, East Lansing, MI, USA aut Li Xingnan Genetics Graduate Program, Michigan State University, 48824, East Lansing, MI, USA aut Shubitowski Dennis Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, 48824, East Lansing, MI, USA aut Samineni Sridhar Department of Food Sciences and Human Nutrition, Michigan State University, East Lansing, MI, USA aut Wills-Karp Marsha Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA aut Ewart Susan Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, 48824, East Lansing, MI, USA aut Immunogenetics Springer-Verlag 58/10(2006-10-01), 851-855 0093-7711 58:10<851 2006 58 251 https://doi.org/10.1007/s00251-006-0146-x text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0146-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ramadas Ravisankar Comparative Medicine and Integrative Biology Program, College of Veterinary Medicine, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Li Xingnan Genetics Graduate Program, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Shubitowski Dennis Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Samineni Sridhar Department of Food Sciences and Human Nutrition, Michigan State University, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Wills-Karp Marsha Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA aut NATIONALLICENCE 700 1- Ewart Susan Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/10(2006-10-01), 851-855 0093-7711 58:10<851 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer