caa a22 4500 467907323 CHVBK 20180406152838.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s00251-006-0087-4 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0087-4 Identification and functional perspective of a novel HLA-A allele: A*0279 [Elektronische Daten] [Fangfang Liu, Shan Wang, Yingjiang Ye, Huagang Zhang, Yu Zhang, Weifeng Chen] A novel HLA-A allele, HLA-A*0279, was identified using PCR-SSP and PCR-SBT methods. It is inheritable. HLA-A*0279 differs from HLA-A*020601 by a single nucleotide at position 497 in exon 3, leading to an amino acid change from Threonine to Isoleucine at the alpha2 helix of HLA molecule. To investigate whether the altered amino acid residue could affect its peptide-binding repertoire, we compared the predicted crystal structure of HLA-A*020601 and HLA-A*0279 by Swiss-PdbViewer software analysis. We found that the crystal structure of the two molecules is very similar except for a difference in the number of hydrogen bonds they can possibly form, which in turn could affect their structural stability. To test whether HLA-A*0279 has the ability to cross-present A*0201 - restricted peptides to T cells, the full lenght cDNA of HLA-A*0201, -A* 020601 and -A*0279 were respectively transfected into COS-7 cells, which were then used as targets in IFN-gamma release Elispot assay. A*2079 was found to be able to present A*0201- restricted peptides to and induce the response of CTL, thus it can be classified as member of the HLA-A2 functional supertype family. This finding would benefit the design of peptide vaccines to be applied in broader populations. Springer-Verlag, 2006 HLA-A supertype nationallicence Cytotoxic T lymphocytes nationallicence Elispot assay nationallicence Crystal structure nationallicence Liu Fangfang Department of Gastroenterological Surgery, Surgical Oncology Laboratory, People's Hospital, Peking University, 100044, Beijing, People' Republic of China aut Wang Shan Department of Gastroenterological Surgery, Surgical Oncology Laboratory, People's Hospital, Peking University, 100044, Beijing, People' Republic of China aut Ye Yingjiang Department of Gastroenterological Surgery, Surgical Oncology Laboratory, People's Hospital, Peking University, 100044, Beijing, People' Republic of China aut Zhang Huagang Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, 100083, Beijing, People' Republic of China aut Zhang Yu Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, 100083, Beijing, People' Republic of China aut Chen Weifeng Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, 100083, Beijing, People' Republic of China aut Immunogenetics Springer-Verlag 58/5-6(2006-06-01), 339-346 0093-7711 58:5-6<339 2006 58 251 https://doi.org/10.1007/s00251-006-0087-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0087-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Fangfang Department of Gastroenterological Surgery, Surgical Oncology Laboratory, People's Hospital, Peking University, 100044, Beijing, People' Republic of China aut NATIONALLICENCE 700 1- Wang Shan Department of Gastroenterological Surgery, Surgical Oncology Laboratory, People's Hospital, Peking University, 100044, Beijing, People' Republic of China aut NATIONALLICENCE 700 1- Ye Yingjiang Department of Gastroenterological Surgery, Surgical Oncology Laboratory, People's Hospital, Peking University, 100044, Beijing, People' Republic of China aut NATIONALLICENCE 700 1- Zhang Huagang Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, 100083, Beijing, People' Republic of China aut NATIONALLICENCE 700 1- Zhang Yu Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, 100083, Beijing, People' Republic of China aut NATIONALLICENCE 700 1- Chen Weifeng Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, 100083, Beijing, People' Republic of China aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/5-6(2006-06-01), 339-346 0093-7711 58:5-6<339 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer