caa a22 4500 467907358 CHVBK 20180406152838.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s00251-006-0115-4 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0115-4 Modo-UG , a marsupial nonclassical MHC class I locus [Elektronische Daten] [Nicolas Gouin, April Wright, Katarzyna Miska, Zuly Parra, Paul Samollow, Michelle Baker, Robert Miller] Modo-UG is a class I gene located in the MHC of the marsupial Monodelphis domestica, the gray, short-tailed opossum. Modo-UG is expressed as three alternatively spliced mRNA forms, all of which encode a transmembrane form with a short cytoplasmic tail that lacks phosphorylation sites typically found in classical class I molecules. The three alternative mRNAs would encode a full-length form, an isoform lacking the α2 domain, and one lacking both α2 and α3 domains. Genotyping both captive-bred and wild M. domestica from different geographic regions revealed no variation in the residues that make up Modo-UG's peptide-binding groove. Modo-UG's low polymorphism is contrasting to that of a nearby class I locus, Modo-UA1, which has a highly polymorphic peptide-binding region. Absence of functional polymorphism in Modo-UG is therefore not a general feature of opossum class I genes but the result of negative selection. Modo-UG is the first MHC linked marsupial class I to be described that appears to clearly have nonclassical features. Springer-Verlag, 2006 Gouin Nicolas Department of Genetics, Southwest Foundation for Biomedical Research, 78245, San Antonio, TX, USA aut Wright April Department of Biology, The University of New Mexico, 87131, Albuquerque, NM, USA aut Miska Katarzyna USDA-ARS, Animal Parasitic Diseases Laboratory, 10300 Baltimore Avenue, Building 1042 BARC-East, 20705, Beltsville, MD, USA aut Parra Zuly Department of Biology, The University of New Mexico, 87131, Albuquerque, NM, USA aut Samollow Paul Department of Veterinary Integrative Biosciences, Texas A&M University, 77843, College Station, TX, USA aut Baker Michelle Department of Biology, The University of New Mexico, 87131, Albuquerque, NM, USA aut Miller Robert Department of Biology, The University of New Mexico, 87131, Albuquerque, NM, USA aut Immunogenetics Springer-Verlag 58/5-6(2006-06-01), 396-406 0093-7711 58:5-6<396 2006 58 251 https://doi.org/10.1007/s00251-006-0115-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0115-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gouin Nicolas Department of Genetics, Southwest Foundation for Biomedical Research, 78245, San Antonio, TX, USA aut NATIONALLICENCE 700 1- Wright April Department of Biology, The University of New Mexico, 87131, Albuquerque, NM, USA aut NATIONALLICENCE 700 1- Miska Katarzyna USDA-ARS, Animal Parasitic Diseases Laboratory, 10300 Baltimore Avenue, Building 1042 BARC-East, 20705, Beltsville, MD, USA aut NATIONALLICENCE 700 1- Parra Zuly Department of Biology, The University of New Mexico, 87131, Albuquerque, NM, USA aut NATIONALLICENCE 700 1- Samollow Paul Department of Veterinary Integrative Biosciences, Texas A&M University, 77843, College Station, TX, USA aut NATIONALLICENCE 700 1- Baker Michelle Department of Biology, The University of New Mexico, 87131, Albuquerque, NM, USA aut NATIONALLICENCE 700 1- Miller Robert Department of Biology, The University of New Mexico, 87131, Albuquerque, NM, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/5-6(2006-06-01), 396-406 0093-7711 58:5-6<396 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer