caa a22 4500 467907374 CHVBK 20180406152838.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s00251-006-0107-4 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0107-4 Isolation of major histocompatibility complex Class I genes from the tammar wallaby ( Macropus eugenii ) [Elektronische Daten] [Hannah Siddle, Janine Deakin, Michelle Baker, Robert Miller, Katherine Belov] The major histocompatibility complex (MHC) plays an essential role in the adaptive immune system of vertebrates through antigen recognition. Although MHC genes are found in all vertebrates, the MHC region is dynamic and has changed throughout vertebrate evolution, making it an important tool for comparative genomics. Marsupials occupy an important position in mammalian phylogeny, yet the MHC of few marsupials has been studied in detail. We report the isolation and analysis of expressed MHC Class I genes from the tammar wallaby, a model marsupial used extensively for the study of mammalian reproduction, genetics, and immunology. We determined that there are at least 11 Class I loci in the tammar genome and isolated six expressed Class I sequences from spleen and testes cDNA libraries, representing at least four loci. Two of the Class I sequences contain substitutions at sites known to be important for antigen binding, perhaps impacting their ability to bind peptides, or the types of peptide to which they bind. Phylogenetic analysis of tammar wallaby Class I sequences and other mammalian Class I sequences suggests that some tammar wallaby and red-necked wallaby loci evolved from common ancestral genes. Springer-Verlag, 2006 Siddle Hannah Centre for Advanced Technologies in Animal Genetics and Reproduction, Faculty of Veterinary Science, University of Sydney, Sydney, Australia aut Deakin Janine ARC Centre for Kangaroo Genomics, Research School of Biological Sciences, Australian National University, Canberra, Australia aut Baker Michelle Department of Biology, The University of New Mexico, 87131, Albuquerque, NM, USA aut Miller Robert Department of Biology, The University of New Mexico, 87131, Albuquerque, NM, USA aut Belov Katherine Centre for Advanced Technologies in Animal Genetics and Reproduction, Faculty of Veterinary Science, University of Sydney, Sydney, Australia aut Immunogenetics Springer-Verlag 58/5-6(2006-06-01), 487-493 0093-7711 58:5-6<487 2006 58 251 https://doi.org/10.1007/s00251-006-0107-4 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0107-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Siddle Hannah Centre for Advanced Technologies in Animal Genetics and Reproduction, Faculty of Veterinary Science, University of Sydney, Sydney, Australia aut NATIONALLICENCE 700 1- Deakin Janine ARC Centre for Kangaroo Genomics, Research School of Biological Sciences, Australian National University, Canberra, Australia aut NATIONALLICENCE 700 1- Baker Michelle Department of Biology, The University of New Mexico, 87131, Albuquerque, NM, USA aut NATIONALLICENCE 700 1- Miller Robert Department of Biology, The University of New Mexico, 87131, Albuquerque, NM, USA aut NATIONALLICENCE 700 1- Belov Katherine Centre for Advanced Technologies in Animal Genetics and Reproduction, Faculty of Veterinary Science, University of Sydney, Sydney, Australia aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/5-6(2006-06-01), 487-493 0093-7711 58:5-6<487 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer