caa a22 4500 467907382 CHVBK 20180406152838.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s00251-006-0119-0 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0119-0 Molecular genotype identification of the Gallus gallus major histocompatibility complex [Elektronische Daten] [Janet Fulton, Helle Juul-Madsen, Christopher Ashwell, Amy McCarron, James Arthur, Neil O'Sullivan, Robert Taylor Jr] The chicken major histocompatibility complex (MHC) is commonly defined by serologic reactions of erythrocytes with antibodies specific to the highly polymorphic MHC class I (BF) and MHC class IV (BG) antigens. The microsatellite marker LEI0258 is known to be physically located within the MHC, between the BG and BF regions. DNA from various serologically defined MHC haplotypes was amplified by polymerase chain reaction with primers surrounding this marker. Twenty-six distinctive allele sizes were identified. Some serologically well-defined MHC haplotypes shared a common LEI0258 allele size but could be distinguished either by the addition of information from another nearby marker (MCW0371) or by small indels or single nucleotide polymorphism (SNP) differences between the alleles. The association between LEI0258 allele and serologically defined MHC haplotype was very consistent for the same haplotype from multiple sources. Sequence information for the region defined by LEI0258 was obtained for 51 different haplotypes. Two internal repeats whose lengths were 13 and 12bp, respectively, are the primary basis for allelic variability. Allele size variation ranges from 182 to 552bp. Four indels and five SNPs in the surrounding sequence provide additional means for distinguishing alleles. Typing with LEI0258 and MCW0371 will be useful in identifying MHC haplotypes in outbred populations of chickens particularly for the initial development of serological reagents. Springer-Verlag, 2006 Chicken MHC nationallicence Molecular identification nationallicence MHC haplotype nationallicence Microsatellite nationallicence Genotype sequence nationallicence Fulton Janet Hy-Line International, P.O. Box 310, 50063, Dallas Center, IA, USA aut Juul-Madsen Helle Department of Animal Health, Welfare and Nutrition, Danish Institute of Agricultural Sciences, 8830, Tjele, Denmark aut Ashwell Christopher Department of Poultry Science, North Carolina State University, 27695, Raleigh, NC, USA aut McCarron Amy Hy-Line International, P.O. Box 310, 50063, Dallas Center, IA, USA aut Arthur James Hy-Line International, P.O. Box 310, 50063, Dallas Center, IA, USA aut O'Sullivan Neil Hy-Line International, P.O. Box 310, 50063, Dallas Center, IA, USA aut Taylor Jr Robert University of New Hampshire, Kendall Hall, 03824, Durham, NH, USA aut Immunogenetics Springer-Verlag 58/5-6(2006-06-01), 407-421 0093-7711 58:5-6<407 2006 58 251 https://doi.org/10.1007/s00251-006-0119-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0119-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fulton Janet Hy-Line International, P.O. Box 310, 50063, Dallas Center, IA, USA aut NATIONALLICENCE 700 1- Juul-Madsen Helle Department of Animal Health, Welfare and Nutrition, Danish Institute of Agricultural Sciences, 8830, Tjele, Denmark aut NATIONALLICENCE 700 1- Ashwell Christopher Department of Poultry Science, North Carolina State University, 27695, Raleigh, NC, USA aut NATIONALLICENCE 700 1- McCarron Amy Hy-Line International, P.O. Box 310, 50063, Dallas Center, IA, USA aut NATIONALLICENCE 700 1- Arthur James Hy-Line International, P.O. Box 310, 50063, Dallas Center, IA, USA aut NATIONALLICENCE 700 1- O'Sullivan Neil Hy-Line International, P.O. Box 310, 50063, Dallas Center, IA, USA aut NATIONALLICENCE 700 1- Taylor Jr Robert University of New Hampshire, Kendall Hall, 03824, Durham, NH, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/5-6(2006-06-01), 407-421 0093-7711 58:5-6<407 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer