caa a22 4500 467907412 CHVBK 20180406152838.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s00251-006-0094-5 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0094-5 Major histocompatibility class II genes in rainbow trout ( Oncorhynchus mykiss ) exhibit temperature dependent downregulation [Elektronische Daten] [Suchita Nath, Stephen Kales, Kazuhiro Fujiki, Brian Dixon] Major histocompatibility (MH) class II receptors are expressed on the surface of specialized antigen-presenting cells in vertebrate immune systems. Their function is to present peptides derived from exogenous pathogens to CD4+ T cells. Variation in the level of expression of these genes has been linked to pathogenesis in various diseases. Very little has been published on the function of MH class II receptors in teleost fish to date. In this study, we have produced polyclonal antibodies recognizing MH class II alpha and beta proteins of rainbow trout and employed them to characterize the expression pattern of these genes. Deglycosylation using N-glycosidase F and endoglycosidase H showed that MH class II alpha is glycosylated in rainbow trout. MH class II beta was also found to be glycosylated as reported previously. Results from Northern blotting revealed that the expression of these genes was not affected by exposure of rainbow trout to temperature of 5°C. However, at 2°C, downregulation of MH class II alpha and beta genes was evident at both the mRNA and protein levels as assessed by Northern and Western blotting, respectively. Because MH class II antigens play an important role in generating an immune response to bacterial and fungal pathogens, downregulation of these genes at low temperature could account for the susceptibility of fish to low temperature-related diseases such as bacterial cold-water disease and winter saprolegniosis. Springer-Verlag, 2006 Major histocompatibility gene nationallicence Glycosylation nationallicence Antibody nationallicence Temperature nationallicence Immunosuppression nationallicence Protein nationallicence Gene expression nationallicence Nath Suchita Department of Biology, University of Waterloo, 200 University Ave., N2L 3G1, West Waterloo, ON, Canada aut Kales Stephen Department of Biology, University of Waterloo, 200 University Ave., N2L 3G1, West Waterloo, ON, Canada aut Fujiki Kazuhiro Department of Biology, University of Waterloo, 200 University Ave., N2L 3G1, West Waterloo, ON, Canada aut Dixon Brian Department of Biology, University of Waterloo, 200 University Ave., N2L 3G1, West Waterloo, ON, Canada aut Immunogenetics Springer-Verlag 58/5-6(2006-06-01), 443-453 0093-7711 58:5-6<443 2006 58 251 https://doi.org/10.1007/s00251-006-0094-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0094-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nath Suchita Department of Biology, University of Waterloo, 200 University Ave., N2L 3G1, West Waterloo, ON, Canada aut NATIONALLICENCE 700 1- Kales Stephen Department of Biology, University of Waterloo, 200 University Ave., N2L 3G1, West Waterloo, ON, Canada aut NATIONALLICENCE 700 1- Fujiki Kazuhiro Department of Biology, University of Waterloo, 200 University Ave., N2L 3G1, West Waterloo, ON, Canada aut NATIONALLICENCE 700 1- Dixon Brian Department of Biology, University of Waterloo, 200 University Ave., N2L 3G1, West Waterloo, ON, Canada aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/5-6(2006-06-01), 443-453 0093-7711 58:5-6<443 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer