caa a22 4500 467907471 CHVBK 20180406152838.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s00251-006-0095-4 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0095-4 Genetic variation at chemokine receptor CCR5 in leporids: alteration at the 2nd extracellular domain by gene conversion with CCR2 in Oryctolagus , but not in Sylvilagus and Lepus species [Elektronische Daten] [C. Carmo, P. Esteves, N. Ferrand, W. van der Loo] Whereas in its natural host (Sylvilagus sps.) the effects of myxoma virus infections are benign, in European rabbit (Oryctolagus cuniculus), it causes a highly infectious disease with very high mortality rate, known as myxomatosis. There is evidence that, as with HIV-1 virus in human, myxoma virus may use chemokine receptors such as CCR5 of the host target cell for entry and activation of pathways of immune avoidance. We have characterized and compared CCR5 genes of leporid species with different susceptibility levels to myxomatosis. The CCR5 protein of O. cuniculus differs markedly from all those known from other species. The most striking was the replacement of a specific peptide motif of the second extracellular loop (ECL2) by a motif, which in other species characterizes the CCR2 molecules. While absent in Sylvilagus and Lepus species, this CCR2 imposed CCR5-ECL2 alteration was observed in all genomes of 25 European rabbits, representing the subspecies O. cuniculus algirus and O. cuniculus cuniculus. Allelic variation at the rabbit CCR5 locus confirmed that the gene conversion predates the subspecies split (1-2Ma). Springer-Verlag, 2006 Chemokine receptors nationallicence Host parasite interaction nationallicence Gene conversion nationallicence Myxoma virus nationallicence Leporidae nationallicence Oryctolagus nationallicence Carmo C. Departamento de Zoologia e Antropologia-, Faculdade de Ciências da Universidade do Porto, Porto, Portugal aut Esteves P. Centro de Investigação em Biodiversidade e Recursos Genéticos (CIBIO), ICETA-UP, Campus Agrário de Vairão Rua Padre Armando Quintas, 4485-661, Vairão, Portugal aut Ferrand N. Centro de Investigação em Biodiversidade e Recursos Genéticos (CIBIO), ICETA-UP, Campus Agrário de Vairão Rua Padre Armando Quintas, 4485-661, Vairão, Portugal aut van der Loo W. Centro de Investigação em Biodiversidade e Recursos Genéticos (CIBIO), ICETA-UP, Campus Agrário de Vairão Rua Padre Armando Quintas, 4485-661, Vairão, Portugal aut Immunogenetics Springer-Verlag 58/5-6(2006-06-01), 494-501 0093-7711 58:5-6<494 2006 58 251 https://doi.org/10.1007/s00251-006-0095-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0095-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Carmo C. Departamento de Zoologia e Antropologia-, Faculdade de Ciências da Universidade do Porto, Porto, Portugal aut NATIONALLICENCE 700 1- Esteves P. Centro de Investigação em Biodiversidade e Recursos Genéticos (CIBIO), ICETA-UP, Campus Agrário de Vairão Rua Padre Armando Quintas, 4485-661, Vairão, Portugal aut NATIONALLICENCE 700 1- Ferrand N. Centro de Investigação em Biodiversidade e Recursos Genéticos (CIBIO), ICETA-UP, Campus Agrário de Vairão Rua Padre Armando Quintas, 4485-661, Vairão, Portugal aut NATIONALLICENCE 700 1- van der Loo W. Centro de Investigação em Biodiversidade e Recursos Genéticos (CIBIO), ICETA-UP, Campus Agrário de Vairão Rua Padre Armando Quintas, 4485-661, Vairão, Portugal aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/5-6(2006-06-01), 494-501 0093-7711 58:5-6<494 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer