caa a22 4500 46790748X CHVBK 20180406152838.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s00251-006-0109-2 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0109-2 Genomic structure, promoter analysis, and expression of the porcine ( Sus scrofa ) Mx1 gene [Elektronische Daten] [Anne Thomas, Melanie Palm, Aurore Broers, Hussein Zezafoun, Daniel Desmecht] Allelic polymorphisms at the mouse Mx1 locus affect the probability of survival after experimental influenzal disease, raising the possibility that marker-assisted selection using the homologous locus could improve the innate resistance of pigs to natural influenza infections. Several issues need to be resolved before efficient large scale screening of the allelic polymorphism at the porcine (Sus scrofa) Mx1 locus can be implemented. First, the Mx1 genomic structure has to be established and sufficient flanking intronic sequences have to be gathered to enable simple PCR amplification of the coding portions of the gene. Then, a basic knowledge of the promoter region needs to be obtained as an allelic variation there can significantly alter absolute levels and/or tissue-specificity of MX protein expression. The results gathered here show that the porcine Mx1 gene and promoter share the major structural and functional characteristics displayed by their homologs described in cattle, mouse, chicken, and man. The crucial function of the proximal interferon-sensitive response elements motif for gene expression is also demonstrated. The sequence data compiled here will allow an extensive analysis of the polymorphisms present among the widest spectrum possible of porcine breeds with the aim to identify an Mx1 allele providing antiviral resistance. Springer-Verlag, 2006 Pig nationallicence Mx nationallicence Influenza nationallicence ISRE nationallicence Interferon nationallicence Thomas Anne Department of Pathology, Faculty of Veterinary Medicine, University of Liege, Sart Tilman B43, 4000, Liège, Belgium aut Palm Melanie Department of Pathology, Faculty of Veterinary Medicine, University of Liege, Sart Tilman B43, 4000, Liège, Belgium aut Broers Aurore Department of Pathology, Faculty of Veterinary Medicine, University of Liege, Sart Tilman B43, 4000, Liège, Belgium aut Zezafoun Hussein Department of Pathology, Faculty of Veterinary Medicine, University of Liege, Sart Tilman B43, 4000, Liège, Belgium aut Desmecht Daniel Department of Pathology, Faculty of Veterinary Medicine, University of Liege, Sart Tilman B43, 4000, Liège, Belgium aut Immunogenetics Springer-Verlag 58/5-6(2006-06-01), 383-389 0093-7711 58:5-6<383 2006 58 251 https://doi.org/10.1007/s00251-006-0109-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0109-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Thomas Anne Department of Pathology, Faculty of Veterinary Medicine, University of Liege, Sart Tilman B43, 4000, Liège, Belgium aut NATIONALLICENCE 700 1- Palm Melanie Department of Pathology, Faculty of Veterinary Medicine, University of Liege, Sart Tilman B43, 4000, Liège, Belgium aut NATIONALLICENCE 700 1- Broers Aurore Department of Pathology, Faculty of Veterinary Medicine, University of Liege, Sart Tilman B43, 4000, Liège, Belgium aut NATIONALLICENCE 700 1- Zezafoun Hussein Department of Pathology, Faculty of Veterinary Medicine, University of Liege, Sart Tilman B43, 4000, Liège, Belgium aut NATIONALLICENCE 700 1- Desmecht Daniel Department of Pathology, Faculty of Veterinary Medicine, University of Liege, Sart Tilman B43, 4000, Liège, Belgium aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/5-6(2006-06-01), 383-389 0093-7711 58:5-6<383 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer