caa a22 4500 467907528 CHVBK 20180406152839.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s00251-006-0080-y doi (NATIONALLICENCE)springer-10.1007/s00251-006-0080-y Diversity and duplication of DQB and DRB -like genes of the MHC in baleen whales (suborder: Mysticeti ) [Elektronische Daten] [C. Baker, M. Vant, M. Dalebout, G. Lento, S. O'Brien, N. Yuhki] The molecular diversity and phylogenetic relationships of two class II genes of the baleen whale major histocompatibility complex were investigated and compared to toothed whales and out-groups. Amplification of the DQB exon 2 provided sequences showing high within-species and between-species nucleotide diversity and uninterrupted reading frames consistent with functional class II loci found in related mammals (e.g., ruminants). Cloning of amplified products indicated gene duplication in the humpback whale and triplication in the southern right whale, with average nucleotide diversity of 5.9 and 6.3%, respectively, for alleles of each species. Significantly higher nonsynonymous divergence at sites coding for peptide binding (32% for humpback and 40% for southern right) suggested that these loci were subject to positive (overdominant) selection. A population survey of humpback whales detected 23 alleles, differing by up to 21% of their inferred amino acid sequences. Amplification of the DRB exon 2 resulted in two groups of sequences. One was most similar to the DRB3 of the cow and present in all whales screened to date, including toothed whales. The second was most similar to the DRB2 of the cow and was found only in the bowhead and right whales. Both loci showed low diversity among species and apparent loss of function or altered function including interruption of reading frames. Finally, comparison of inferred protein sequence of the DRB3-like locus suggested convergence with the DQB, perhaps resulting from intergenic conversion or recombination. Springer-Verlag, 2006 Overdominant selection nationallicence Diversity nationallicence Cetaceans nationallicence Dolphins nationallicence Baker C. School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand aut Vant M. School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand aut Dalebout M. School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand aut Lento G. School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand aut O'Brien S. Laboratory of Genomic Diversity, National Cancer Institute, Frederick, MD, USA aut Yuhki N. Laboratory of Genomic Diversity, National Cancer Institute, Frederick, MD, USA aut Immunogenetics Springer-Verlag 58/4(2006-05-01), 283-296 0093-7711 58:4<283 2006 58 251 https://doi.org/10.1007/s00251-006-0080-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0080-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baker C. School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand aut NATIONALLICENCE 700 1- Vant M. School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand aut NATIONALLICENCE 700 1- Dalebout M. School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand aut NATIONALLICENCE 700 1- Lento G. School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand aut NATIONALLICENCE 700 1- O'Brien S. Laboratory of Genomic Diversity, National Cancer Institute, Frederick, MD, USA aut NATIONALLICENCE 700 1- Yuhki N. Laboratory of Genomic Diversity, National Cancer Institute, Frederick, MD, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/4(2006-05-01), 283-296 0093-7711 58:4<283 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer