caa a22 4500 467907536 CHVBK 20180406152839.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s00251-005-0074-1 doi (NATIONALLICENCE)springer-10.1007/s00251-005-0074-1 At least one YMHCI molecule in the chicken is alloimmunogenic and dynamically expressed on spleen cells during development [Elektronische Daten] [Henry Hunt, Ronald Goto, Douglas Foster, Larry Bacon, Marcia Miller] Transcriptionally active, MHC class I (MHCI) loci are located in two separate polymorphic genomic regions in the chicken called B and Y. The YMHCI gene sequences encode molecules with uncommon substitutions in the antigen-binding region indicating that YMHCI molecules are likely unique and may bind a specialized form of antigen distinct from that of other antigen-binding MHCI molecules. To learn whether YMHCI gene expression results in the production of alloantigens at the cell surface, we immunized 15I5 × 7 2 chickens using syngeneic RP9 cells expressing transduced YF1w*7.1, a potentially alloimmunogenic YMHCI allele from the Y7 haplotype present in line C. The resulting antisera show that YF1w*7.1 MHCI molecules are immunogenic and expressed on the surfaces of cells in blood and spleen of line C chickens. Virtually all CD3+, CD4+, and CD8+ cells circulating in line C blood are positive, as are BU1+ cells. The YF1w*7.1 MHCI allele is dynamically expressed at levels comparable to but transcriptionally independent of classical BMHCI on erythrocytes, lymphocytes, granulocytes, monocytes, and thrombocytes within the spleen pre- and post-hatching. The antisera react with cells from two among four haplotypes segregating in closed populations of lines N and P. YMHCI shares features associated with both classical and non-classical MHCI. It is becoming increasingly likely that YMHCI has a fundamental role in avian immunity and thereby needs to be included in the growing spectrum of functionally active, diverse MHCI molecules no longer adequately described by the classical/non-classical dichotomy. Springer-Verlag, 2006 Chicken major histocompatibility complex nationallicence MHCI nationallicence Alloantigens nationallicence MHC Y (formerly Rfp-Y ) nationallicence MHC B nationallicence Hunt Henry Avian Disease and Oncology Laboratory, Agricultural Research Service, USDA, 3606 East Mount Hope Road, 48823, East Lansing, MI, USA aut Goto Ronald Division of Molecular Biology, Beckman Research Institute, City of Hope National Medical Center, 1450 East Duarte Road, 91010-3011, Duarte, CA, USA aut Foster Douglas Department of Animal Science, University of Minnesota, 55108, St. Paul, MN, USA aut Bacon Larry Avian Disease and Oncology Laboratory, Agricultural Research Service, USDA, 3606 East Mount Hope Road, 48823, East Lansing, MI, USA aut Miller Marcia Division of Molecular Biology, Beckman Research Institute, City of Hope National Medical Center, 1450 East Duarte Road, 91010-3011, Duarte, CA, USA aut Immunogenetics Springer-Verlag 58/4(2006-05-01), 297-307 0093-7711 58:4<297 2006 58 251 https://doi.org/10.1007/s00251-005-0074-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-005-0074-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hunt Henry Avian Disease and Oncology Laboratory, Agricultural Research Service, USDA, 3606 East Mount Hope Road, 48823, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Goto Ronald Division of Molecular Biology, Beckman Research Institute, City of Hope National Medical Center, 1450 East Duarte Road, 91010-3011, Duarte, CA, USA aut NATIONALLICENCE 700 1- Foster Douglas Department of Animal Science, University of Minnesota, 55108, St. Paul, MN, USA aut NATIONALLICENCE 700 1- Bacon Larry Avian Disease and Oncology Laboratory, Agricultural Research Service, USDA, 3606 East Mount Hope Road, 48823, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Miller Marcia Division of Molecular Biology, Beckman Research Institute, City of Hope National Medical Center, 1450 East Duarte Road, 91010-3011, Duarte, CA, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/4(2006-05-01), 297-307 0093-7711 58:4<297 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer