caa a22 4500 467907544 CHVBK 20180406152839.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s00251-005-0076-z doi (NATIONALLICENCE)springer-10.1007/s00251-005-0076-z HLA-E, HLA-F, and HLA-G polymorphism: genomic sequence defines haplotype structure and variation spanning the nonclassical class I genes [Elektronische Daten] [Chul-Woo Pyo, Luke Williams, Yuki Moore, Hironobu Hyodo, Shuying Li, Lue Zhao, Noriko Sageshima, Akiko Ishitani, Daniel Geraghty] Despite several studies that defined the polymorphism of the nonclassical human leukocyte antigen-E (HLA-E), HLA-F, and HLA-G genes, most polymorphisms thus far examined in correlative studies were derived from the coding sequences of these genes. In addition, some discrepancies and ambiguities in the available data have persisted in current databases. To expand the data available and to resolve some of the discrepant data, we have defined protocols that allow for the amplification of 6 to 7kb of contiguous genomic sequence for each gene, including all of the coding and intron sequences, approximately 2kb of 5' flanking promoter sequence, and 1kb of 3' flanking sequence. Using long-range polymerase chain reaction (PCR) protocols, generating either one or two PCR products depending on the locus, amplified genomic DNA was directly sequenced to completion using a set of about 30 primers over each locus to yield contiguous sequence data from both strands. Using this approach, we sequenced 33 genomic DNAs, from Asian, African American, and Caucasian samples. The results of this analysis confirmed several previously reported coding sequence variants, identified several new allelic variants, and also defined extensive variation in intron and flanking sequences. It was possible to construct haplotype maps and to identify tagging single nucleotide polymorphisms that can be used to detect the composite variation spanning all three genes. Springer-Verlag, 2006 MHC nationallicence Nonclassical class I nationallicence Genomics nationallicence SNPs nationallicence Alleles nationallicence Pyo Chul-Woo The Clinical Research Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave., 98109-1024, N. Seattle, WA, USA aut Williams Luke The Clinical Research Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave., 98109-1024, N. Seattle, WA, USA aut Moore Yuki The Clinical Research Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave., 98109-1024, N. Seattle, WA, USA aut Hyodo Hironobu The Clinical Research Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave., 98109-1024, N. Seattle, WA, USA aut Li Shuying The Public Health Sciences Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave., 98109-1024, N. Seattle, WA, USA aut Zhao Lue The Public Health Sciences Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave., 98109-1024, N. Seattle, WA, USA aut Sageshima Noriko Nara Medical University, 634, Kashihara, Nara, Japan aut Ishitani Akiko Nara Medical University, 634, Kashihara, Nara, Japan aut Geraghty Daniel The Clinical Research Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave., 98109-1024, N. Seattle, WA, USA aut Immunogenetics Springer-Verlag 58/4(2006-05-01), 241-251 0093-7711 58:4<241 2006 58 251 https://doi.org/10.1007/s00251-005-0076-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-005-0076-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pyo Chul-Woo The Clinical Research Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave., 98109-1024, N. Seattle, WA, USA aut NATIONALLICENCE 700 1- Williams Luke The Clinical Research Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave., 98109-1024, N. Seattle, WA, USA aut NATIONALLICENCE 700 1- Moore Yuki The Clinical Research Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave., 98109-1024, N. Seattle, WA, USA aut NATIONALLICENCE 700 1- Hyodo Hironobu The Clinical Research Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave., 98109-1024, N. Seattle, WA, USA aut NATIONALLICENCE 700 1- Li Shuying The Public Health Sciences Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave., 98109-1024, N. Seattle, WA, USA aut NATIONALLICENCE 700 1- Zhao Lue The Public Health Sciences Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave., 98109-1024, N. Seattle, WA, USA aut NATIONALLICENCE 700 1- Sageshima Noriko Nara Medical University, 634, Kashihara, Nara, Japan aut NATIONALLICENCE 700 1- Ishitani Akiko Nara Medical University, 634, Kashihara, Nara, Japan aut NATIONALLICENCE 700 1- Geraghty Daniel The Clinical Research Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave., 98109-1024, N. Seattle, WA, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/4(2006-05-01), 241-251 0093-7711 58:4<241 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer