caa a22 4500 467907560 CHVBK 20180406152839.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s00251-006-0102-9 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0102-9 Study of Cynomolgus monkey (Macaca fascicularis) MhcDRB (Mafa-DRB) polymorphism in two populations [Elektronische Daten] [Antoine Blancher, Pierre Tisseyre, Marianne Dutaur, Pol-André Apoil, Claudine Maurer, Valérie Quesniaux, Friedrich Raulf, Marc Bigaud, Michel Abbal] Cynomolgus monkey is one of the macaque species currently used as an animal model for experimental surgery and medicine, in particular, to experiment new drugs or therapy protocols designed for the prevention of allograft rejection. In this field, it is of utmost importance to select histoincompatible recipient-donor pairs. One way to ensure incompatibility between donor and recipient is to check their major histocompatibility complex (MHC) genotypes at the loci playing a determinant role in histocompatibility. We report in this paper on the cynomolgus monkey DRB polymorphism evidenced by sequencing of amplified exon 2 separated either by denaturing gradient gel electrophoresis (DGGE), or by cloning. By the study of 253 unrelated animals from two populations (Mauritius and The Philippines), we characterized 50 exon 2 sequences among which 28 were identical to sequences already reported in Macaca fascicularis or other macaque species (Macaca mulatta, Macaca nemestrina). By cloning and sequencing DRB cDNA, we revealed two additional DRB alleles. Out of the 20 haplotypes that we defined here, only two were found in both populations. The functional impact of DR incompatibility was studied in vitro by mixed lymphocyte culture. Springer-Verlag, 2006 Blancher Antoine Laboratoire d'Immunogenetique moleculaire, Universite Paul Sabatier, Faculte de Medecine de Rangueil, Batiment A2, 133, Route de Narbonne, 31062, Toulouse cedex 4, France aut Tisseyre Pierre Laboratoire d'Immunogenetique moleculaire, Universite Paul Sabatier, Faculte de Medecine de Rangueil, Batiment A2, 133, Route de Narbonne, 31062, Toulouse cedex 4, France aut Dutaur Marianne Laboratoire d'Immunogenetique moleculaire, Universite Paul Sabatier, Faculte de Medecine de Rangueil, Batiment A2, 133, Route de Narbonne, 31062, Toulouse cedex 4, France aut Apoil Pol-André Laboratoire d'Immunogenetique moleculaire, Universite Paul Sabatier, Faculte de Medecine de Rangueil, Batiment A2, 133, Route de Narbonne, 31062, Toulouse cedex 4, France aut Maurer Claudine Transplantation Research, Novartis Pharma AG, WSJ-386.1.55A, CH-4002, Basel, Switzerland aut Quesniaux Valérie Institut de Transgénose, CNRS-GEM FRE 2358, 3B, rue de la Férollerie, F-45071, Orléans, cedex 2, France aut Raulf Friedrich Transplantation Research, Novartis Pharma AG, WSJ-386.1.55A, CH-4002, Basel, Switzerland aut Bigaud Marc Transplantation Research, Novartis Pharma AG, WSJ-386.1.55A, CH-4002, Basel, Switzerland aut Abbal Michel Laboratoire d'Immunogenetique moleculaire, Universite Paul Sabatier, Faculte de Medecine de Rangueil, Batiment A2, 133, Route de Narbonne, 31062, Toulouse cedex 4, France aut Immunogenetics Springer-Verlag 58/4(2006-05-01), 269-282 0093-7711 58:4<269 2006 58 251 https://doi.org/10.1007/s00251-006-0102-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0102-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Blancher Antoine Laboratoire d'Immunogenetique moleculaire, Universite Paul Sabatier, Faculte de Medecine de Rangueil, Batiment A2, 133, Route de Narbonne, 31062, Toulouse cedex 4, France aut NATIONALLICENCE 700 1- Tisseyre Pierre Laboratoire d'Immunogenetique moleculaire, Universite Paul Sabatier, Faculte de Medecine de Rangueil, Batiment A2, 133, Route de Narbonne, 31062, Toulouse cedex 4, France aut NATIONALLICENCE 700 1- Dutaur Marianne Laboratoire d'Immunogenetique moleculaire, Universite Paul Sabatier, Faculte de Medecine de Rangueil, Batiment A2, 133, Route de Narbonne, 31062, Toulouse cedex 4, France aut NATIONALLICENCE 700 1- Apoil Pol-André Laboratoire d'Immunogenetique moleculaire, Universite Paul Sabatier, Faculte de Medecine de Rangueil, Batiment A2, 133, Route de Narbonne, 31062, Toulouse cedex 4, France aut NATIONALLICENCE 700 1- Maurer Claudine Transplantation Research, Novartis Pharma AG, WSJ-386.1.55A, CH-4002, Basel, Switzerland aut NATIONALLICENCE 700 1- Quesniaux Valérie Institut de Transgénose, CNRS-GEM FRE 2358, 3B, rue de la Férollerie, F-45071, Orléans, cedex 2, France aut NATIONALLICENCE 700 1- Raulf Friedrich Transplantation Research, Novartis Pharma AG, WSJ-386.1.55A, CH-4002, Basel, Switzerland aut NATIONALLICENCE 700 1- Bigaud Marc Transplantation Research, Novartis Pharma AG, WSJ-386.1.55A, CH-4002, Basel, Switzerland aut NATIONALLICENCE 700 1- Abbal Michel Laboratoire d'Immunogenetique moleculaire, Universite Paul Sabatier, Faculte de Medecine de Rangueil, Batiment A2, 133, Route de Narbonne, 31062, Toulouse cedex 4, France aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/4(2006-05-01), 269-282 0093-7711 58:4<269 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer