caa a22 4500 467907625 CHVBK 20180406152839.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s00251-006-0122-5 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0122-5 Differential TCR gene usage between WC1 − and WC1 + ruminant γδ T cell subpopulations including those responding to bacterial antigen [Elektronische Daten] [Seth Blumerman, Carolyn Herzig, Aric Rogers, Janice Telfer, Cynthia Baldwin] Ruminant γδ T cells are divided into subpopulations based on the presence or absence of WC1 co-receptors (scavenger-receptor-cysteine-rich family members uniquely expressed on γδ T cells). Evidence suggests WC1+ are inflammatory while WC1− are regulatory and that they also differ in their tissue distribution. Recently, this paradigm was refined further as cells that produce interferon-γ and proliferate to autologous antigens, leptospira antigens, or IL-12 were largely found within the WC1+ subpopulation that bears the WC1.1 antigenic epitope but not that bearing the WC1.2 epitope. Here, the T cell receptor gene expression by these different subpopulations (WC1−, WC1.1+, and WC1.2+) was compared using flow cytometrically-purified cells and reverse transcriptase-polymerase chain reaction (RT-PCR). The WC1− γδ T cells had transcripts for all 11 possible combinations of the TRG subgroup V and C genes while those in both WC1+ subpopulations were restricted to TRGV3-TRGC5 and TRGV7-TRGC5. In contrast, all three subpopulations expressed transcripts from all four known bovine TRDV genes. Further analysis of the WC1+ γδ T cells that proliferated in leptospira antigen-stimulated cultures indicated that they do not represent a unique subpopulation within the larger WC1+ population based on their TCR gene usage. Moreover, sequencing of 65 transcripts showed that their junctional regions were diverse as TRGJ5-1, TRGJ5-2, TRDJ1, and TRDJ3 were used, and CDR3s ranged from 9 to 24 amino acids. The restricted but shared γδ TCR gene usage for WC1.1 + , WC1.2+, and WC1+-antigen-responsive cells leaves open the possibility that the WC1 co-receptor is an important determining element in the activation process and subsequent response. Springer-Verlag, 2006 γδ T cells nationallicence γδ T cell receptor nationallicence γδ T cell receptor genes nationallicence WC1 nationallicence Ruminant nationallicence Blumerman Seth Department of Veterinary and Animal Sciences, University of Massachusetts, 01003, Amherst, MA, USA aut Herzig Carolyn Department of Veterinary and Animal Sciences, University of Massachusetts, 01003, Amherst, MA, USA aut Rogers Aric Program in Molecular and Cellular Biology, University of Massachusetts, 01003, Amherst, MA, USA aut Telfer Janice Department of Veterinary and Animal Sciences, University of Massachusetts, 01003, Amherst, MA, USA aut Baldwin Cynthia Department of Veterinary and Animal Sciences, University of Massachusetts, 01003, Amherst, MA, USA aut Immunogenetics Springer-Verlag 58/8(2006-08-01), 680-692 0093-7711 58:8<680 2006 58 251 https://doi.org/10.1007/s00251-006-0122-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0122-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Blumerman Seth Department of Veterinary and Animal Sciences, University of Massachusetts, 01003, Amherst, MA, USA aut NATIONALLICENCE 700 1- Herzig Carolyn Department of Veterinary and Animal Sciences, University of Massachusetts, 01003, Amherst, MA, USA aut NATIONALLICENCE 700 1- Rogers Aric Program in Molecular and Cellular Biology, University of Massachusetts, 01003, Amherst, MA, USA aut NATIONALLICENCE 700 1- Telfer Janice Department of Veterinary and Animal Sciences, University of Massachusetts, 01003, Amherst, MA, USA aut NATIONALLICENCE 700 1- Baldwin Cynthia Department of Veterinary and Animal Sciences, University of Massachusetts, 01003, Amherst, MA, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/8(2006-08-01), 680-692 0093-7711 58:8<680 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer