caa a22 4500 467907633 CHVBK 20180406152839.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s00251-006-0137-y doi (NATIONALLICENCE)springer-10.1007/s00251-006-0137-y Generation and maintenance of diversity in the cattle MHC class I region [Elektronische Daten] [James Birch, Lisa Murphy, Niall MacHugh, Shirley Ellis] Major histocompatibility complex (MHC) class I genes play a crucial role in the immune defence against intracellular pathogens. An important evolutionary strategy is to generate and maintain a high level of diversity in these genes. Humans express three highly polymorphic classical MHC class I genes (HLA-A, HLA-B and HLA-C). In contrast, some species, for example rat and rhesus macaque, maintain diversity by generation of haplotypes that vary considerably with regard to the number and combination of transcribed genes. Cattle appear to use both strategies. We show that various combinations of six apparently classical genes, three of which are highly polymorphic, are transcribed on different haplotypes. Although additional sequences were identified in both cDNA and gDNA, it was not possible to assign them to any of these defined genes. Most were highly divergent or were non-classical class I genes. Thus, we found little evidence for frequent duplication and deletion of classical class I genes as reported in some other species. However, the maintenance of class I diversity in cattle may involve limited gene shuffling and deletion, possibly as a result of unequal crossing-over within the class I region. Springer-Verlag, 2006 MHC nationallicence Cattle class I genes nationallicence Haplotype diversity nationallicence Birch James Immunology Division, Institute for Animal Health, RG20 7NN, Compton, UK aut Murphy Lisa Division of Animal Production and Public Health, Institute for Comparative Medicine, Faculty of Veterinary Medicine, Glasgow University, Bearsden Road, G61 1QH, Glasgow, UK aut MacHugh Niall The Centre for Tropical Veterinary Medicine, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, EH25 9RG, Roslin, Midlothian, UK aut Ellis Shirley Immunology Division, Institute for Animal Health, RG20 7NN, Compton, UK aut Immunogenetics Springer-Verlag 58/8(2006-08-01), 670-679 0093-7711 58:8<670 2006 58 251 https://doi.org/10.1007/s00251-006-0137-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0137-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Birch James Immunology Division, Institute for Animal Health, RG20 7NN, Compton, UK aut NATIONALLICENCE 700 1- Murphy Lisa Division of Animal Production and Public Health, Institute for Comparative Medicine, Faculty of Veterinary Medicine, Glasgow University, Bearsden Road, G61 1QH, Glasgow, UK aut NATIONALLICENCE 700 1- MacHugh Niall The Centre for Tropical Veterinary Medicine, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, EH25 9RG, Roslin, Midlothian, UK aut NATIONALLICENCE 700 1- Ellis Shirley Immunology Division, Institute for Animal Health, RG20 7NN, Compton, UK aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/8(2006-08-01), 670-679 0093-7711 58:8<670 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer