caa a22 4500 46790765X CHVBK 20180406152839.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s00251-006-0153-y doi (NATIONALLICENCE)springer-10.1007/s00251-006-0153-y Two factor H-related proteins from the mouse: expression analysis and functional characterization [Elektronische Daten] [Jens Hellwage, Florian Eberle, Tanja Babuke, Harald Seeberger, Heiko Richter, Anja Kunert, Albert Härtl, Peter Zipfel, T. Jokiranta, Mihály Józsi] Complement factor H-related (FHR) proteins display structural and functional similarities to each other and to the complement regulator factor H (FH). FHRs have been identified in various species, including human, rat, and the fish barred sand bass. As mice provide a useful model system to study the physiological role of FHRs in vivo, we aimed at characterizing murine FHR proteins. Two putative FHRs of approximately 100 and 38kDa were detected in mouse plasma using FH-specific antiserum. In a liver cDNA library, three murine FHR-encoding transcripts were identified. Two clones code for related FHR proteins termed FHR-C and FHR-C_v1, which in secreted form are composed of 14 and 13 short consensus repeat (SCR) domains, homologous to SCRs 6-17 and 19-20 of FH. The third transcript, FHR-B, is derived from a separate gene and codes for a secreted protein composed of five SCR domains. FHR-B displays homology to SCRs 5-7 and 19-20 of FH. Expression of FHR-B in various tissues was analyzed by real-time polymerase chain reaction and was identified at high levels in liver, kidney and heart. In liver, FHR-B transcript level was even higher than that of FH. In addition, FHR-B was expressed as a recombinant 37-kDa protein, and this recombinant FHR-B interacted with the ligands heparin and human C3b. Using mouse plasma, the native presumptive FHR proteins were also analyzed in binding assays. In summary, we identify two FHR proteins in mice and for the first time characterize a murine FHR as a heparin- and C3b-binding protein. Springer-Verlag, 2006 Factor H-related nationallicence Complement nationallicence Mouse nationallicence Hellwage Jens Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, Jena, Germany aut Eberle Florian Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, Jena, Germany aut Babuke Tanja Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, Jena, Germany aut Seeberger Harald Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, Jena, Germany aut Richter Heiko Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, Jena, Germany aut Kunert Anja Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, Jena, Germany aut Härtl Albert Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, Jena, Germany aut Zipfel Peter Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, Jena, Germany aut Jokiranta T. Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Helsinki, Finland aut Józsi Mihály Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, Jena, Germany aut Immunogenetics Springer-Verlag 58/11(2006-11-01), 883-893 0093-7711 58:11<883 2006 58 251 https://doi.org/10.1007/s00251-006-0153-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0153-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hellwage Jens Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, Jena, Germany aut NATIONALLICENCE 700 1- Eberle Florian Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, Jena, Germany aut NATIONALLICENCE 700 1- Babuke Tanja Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, Jena, Germany aut NATIONALLICENCE 700 1- Seeberger Harald Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, Jena, Germany aut NATIONALLICENCE 700 1- Richter Heiko Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, Jena, Germany aut NATIONALLICENCE 700 1- Kunert Anja Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, Jena, Germany aut NATIONALLICENCE 700 1- Härtl Albert Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, Jena, Germany aut NATIONALLICENCE 700 1- Zipfel Peter Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, Jena, Germany aut NATIONALLICENCE 700 1- Jokiranta T. Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Helsinki, Finland aut NATIONALLICENCE 700 1- Józsi Mihály Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, Jena, Germany aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/11(2006-11-01), 883-893 0093-7711 58:11<883 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer