caa a22 4500 467907668 CHVBK 20180406152839.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s00251-006-0150-1 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0150-1 Reassignment of the murine 3′ TRDD1 recombination signal sequence [Elektronische Daten] [C. Touvrey, L. Cowell, A. Lieberman, P. Marche, E. Jouvin-Marche, S. Candéias] T cell receptor genes are assembled in developing T lymphocytes from discrete V, D, and J genes by a site-specific somatic rearrangement mechanism. A flanking recombination signal, composed of a conserved heptamer and a semiconserved nonamer separated by 12 or 23 variable nucleotides, targets the activity of the rearrangement machinery to the adjoining V, D, and J genes. Following the rearrangement of V, D, or J genes, their respective recombination signals are ligated together. Although these signal joints are allegedly invariant, created by the head-to-head abuttal of the heptamers, some do exhibit junctional diversity. Recombination signals were initially identified by comparison and alignment of germ-line sequences with the sequence of rearranged genes. However, their overall low level of sequence conservation makes their characterization solely from sequence data difficult. Recently, computational analysis unraveled correlations between nucleotides at several positions scattered within the spacer and recombination activity, so that it is now possible to identify putative recombination signals and determine and predict their recombination efficiency. In this paper, we analyzed the variability introduced in signal joints generated after rearrangement of the TRDD1 and TRDD2 genes in murine thymocytes. The recurrent presence of identical nucleotides inserted in these signal joints led us to reconsider the location and sequence of the TRDD1 recombination signal. By combining molecular characterization and computational analysis, we show that the functional TRDD1 recombination signal is shifted inside the putative coding sequence of the TRDD1 gene and, consequently, that this gene is shorter than indicated in the databases. Springer-Verlag, 2006 T cell receptor gene nationallicence V(D)J recombination nationallicence Recombination signal nationallicence Signal joint nationallicence Junctional diversity nationallicence Touvrey C. CEA, DSV, DRDC, Laboratoire d'Immunochimie; INSERM U548; UJF, 38054, Grenoble, France aut Cowell L. Departments of Biostatistics and Bioinformatics and Immunology, Duke University Centre for Computational Immunology, Duke University, Durham, NC, USA aut Lieberman A. Departments of Biostatistics and Bioinformatics and Immunology, Duke University Centre for Computational Immunology, Duke University, Durham, NC, USA aut Marche P. CEA, DSV, DRDC, Laboratoire d'Immunochimie; INSERM U548; UJF, 38054, Grenoble, France aut Jouvin-Marche E. CEA, DSV, DRDC, Laboratoire d'Immunochimie; INSERM U548; UJF, 38054, Grenoble, France aut Candéias S. CEA, DSV, DRDC, Laboratoire d'Immunochimie; INSERM U548; UJF, 38054, Grenoble, France aut Immunogenetics Springer-Verlag 58/11(2006-11-01), 895-903 0093-7711 58:11<895 2006 58 251 https://doi.org/10.1007/s00251-006-0150-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0150-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Touvrey C. CEA, DSV, DRDC, Laboratoire d'Immunochimie; INSERM U548; UJF, 38054, Grenoble, France aut NATIONALLICENCE 700 1- Cowell L. Departments of Biostatistics and Bioinformatics and Immunology, Duke University Centre for Computational Immunology, Duke University, Durham, NC, USA aut NATIONALLICENCE 700 1- Lieberman A. Departments of Biostatistics and Bioinformatics and Immunology, Duke University Centre for Computational Immunology, Duke University, Durham, NC, USA aut NATIONALLICENCE 700 1- Marche P. CEA, DSV, DRDC, Laboratoire d'Immunochimie; INSERM U548; UJF, 38054, Grenoble, France aut NATIONALLICENCE 700 1- Jouvin-Marche E. CEA, DSV, DRDC, Laboratoire d'Immunochimie; INSERM U548; UJF, 38054, Grenoble, France aut NATIONALLICENCE 700 1- Candéias S. CEA, DSV, DRDC, Laboratoire d'Immunochimie; INSERM U548; UJF, 38054, Grenoble, France aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/11(2006-11-01), 895-903 0093-7711 58:11<895 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer