caa a22 4500 467907676 CHVBK 20180406152839.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s00251-006-0163-9 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0163-9 An improved RT-PCR method for the detection of killer-cell immunoglobulin-like receptor (KIR) transcripts [Elektronische Daten] [A. Thompson, A. van der Slik, F. Koning, J. van Bergen] Killer cell immunoglobulin-like receptors (KIRs) are expressed on human natural killer (NK) cells and a proportion of T cells. As the specificity of these NK and T cells is, at least in part, determined by the combination of KIRs they express, it is important to be able to determine the KIR expression pattern of NK and T cell clones to understand their function. However, for most KIR genes, specific reagents to detect expression are currently either unavailable or sensitive to allelic variations. In this study, a reverse transcriptase-polymerase chain reaction (RT-PCR) that uses new primer sets for the gene-specific detection of KIR transcripts is presented and validated. The key advantage of this RT-PCR method over previously published ones is that it was designed to detect transcripts of all confirmed allelic variants of the KIR genes, while remaining gene-specific. Springer-Verlag, 2006 Killer-cell immunoglobulin-like receptor (KIR) nationallicence RT-PCR nationallicence Natural killer (NK) cells nationallicence Thompson A. Department of Immunmohematology and Blood Transfusion, Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands aut van der Slik A. Department of Immunmohematology and Blood Transfusion, Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands aut Koning F. Department of Immunmohematology and Blood Transfusion, Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands aut van Bergen J. Department of Immunmohematology and Blood Transfusion, Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands aut Immunogenetics Springer-Verlag 58/11(2006-11-01), 865-872 0093-7711 58:11<865 2006 58 251 https://doi.org/10.1007/s00251-006-0163-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0163-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Thompson A. Department of Immunmohematology and Blood Transfusion, Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands aut NATIONALLICENCE 700 1- van der Slik A. Department of Immunmohematology and Blood Transfusion, Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands aut NATIONALLICENCE 700 1- Koning F. Department of Immunmohematology and Blood Transfusion, Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands aut NATIONALLICENCE 700 1- van Bergen J. Department of Immunmohematology and Blood Transfusion, Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/11(2006-11-01), 865-872 0093-7711 58:11<865 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer