caa a22 4500 467907684 CHVBK 20180406152839.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s00251-006-0151-0 doi (NATIONALLICENCE)springer-10.1007/s00251-006-0151-0 Mhc class I genes of the cichlid fish Oreochromis niloticus [Elektronische Daten] [Akie Sato, Roman Dongak, Li Hao, Naoko Takezaki, Seikou Shintani, Takashi Aoki, Jan Klein] In terms of number of species, perciform (perch-like) fishes are one of the most diversified groups of modern vertebrates. Within this group, the family Cichlidae is best known for its spectacular adaptive radiation in the great lakes of East Africa. The molecular tool kit used in the study of this radiation includes the major histocompatibility complex (Mhc) genes. To refine this tool, information about the organization of the Mhc regions is badly needed. In this study, the first step was taken toward providing such information for the Mhc class one regions of Oreochromis niloticus, a representative species of the tilapiine branch of the Cichlidae, for which good bacterial artificial chromosome library is available. Screening of the library with class I gene probes led to the identification and isolation of 31 class-I-positive clones. Sequencing of one of these clones and partial characterization of the remaining clones for the presence of class I exons resulted in the construction of two contigs representing the class I region of this species as well as identification of seven additional class-I-positive singleton clones. The O. niloticus genome was shown to contain at least 28 class I genes or gene fragments. The shorter of the two contigs was approximately 330kb long and contained eight class I genes/gene fragments; the longer contig encompassed 1,200kb of sequence and contained minimally 17 class I genes/gene fragments; three additional class I genes were found to be borne by a clone that might be part of the shorter contig. Springer-Verlag, 2006 Major histocompatibility complex nationallicence Mhc nationallicence Cichlid fish nationallicence Oreochromis niloticus nationallicence Class I genes nationallicence Sato Akie Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, 230-8501, Yokohama, Japan aut Dongak Roman Max-Planck-Institut für Biologie, Abteilung Immungenetik, Tübingen, Germany aut Hao Li Department of Biology, Pennsylvania State University, 16802, University Park, PA, USA aut Takezaki Naoko Max-Planck-Institut für Biologie, Abteilung Immungenetik, Tübingen, Germany aut Shintani Seikou Max-Planck-Institut für Biologie, Abteilung Immungenetik, Tübingen, Germany aut Aoki Takashi Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Tokyo, Japan aut Klein Jan Max-Planck-Institut für Biologie, Abteilung Immungenetik, Tübingen, Germany aut Immunogenetics Springer-Verlag 58/11(2006-11-01), 917-928 0093-7711 58:11<917 2006 58 251 https://doi.org/10.1007/s00251-006-0151-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0151-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sato Akie Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, 230-8501, Yokohama, Japan aut NATIONALLICENCE 700 1- Dongak Roman Max-Planck-Institut für Biologie, Abteilung Immungenetik, Tübingen, Germany aut NATIONALLICENCE 700 1- Hao Li Department of Biology, Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Takezaki Naoko Max-Planck-Institut für Biologie, Abteilung Immungenetik, Tübingen, Germany aut NATIONALLICENCE 700 1- Shintani Seikou Max-Planck-Institut für Biologie, Abteilung Immungenetik, Tübingen, Germany aut NATIONALLICENCE 700 1- Aoki Takashi Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Tokyo, Japan aut NATIONALLICENCE 700 1- Klein Jan Max-Planck-Institut für Biologie, Abteilung Immungenetik, Tübingen, Germany aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/11(2006-11-01), 917-928 0093-7711 58:11<917 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer