caa a22 4500 467907692 CHVBK 20180406152839.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s00251-006-0145-y doi (NATIONALLICENCE)springer-10.1007/s00251-006-0145-y Characterization of swine leukocyte antigen polymorphism by sequence-based and PCR-SSP methods in Meishan pigs [Elektronische Daten] [Chak-Sum Ho, Erin Rochelle, Gregory Martens, Lawrence Schook, Douglas Smith] Resource herds of swine leukocyte antigen (SLA)-characterized pigs are an important tool for the study of immune responses, disease resistance, and production traits. They are also valuable large animal models for biomedical research, such as transplantation. The Meishan breed of pig is an economically significant breed that is available at several research institutions in the United States. We have characterized the SLA polymorphism of the breeding stock in the herd maintained at the University of Illinois and developed a simple assay to SLA type individuals within that herd. We have used a reverse transcription-polymerase chain reaction (RT-PCR)-based SLA typing method to clone and DNA sequence 19 SLA alleles at three SLA class Ia (SLA-1, SLA-2, and SLA-3) and two SLA class II (SLA-DRB1 and SLA-DQB1) loci. Based on this sequence information, a rapid SLA typing assay was developed to discriminate each allele using PCR with sequence-specific primers (PCR-SSP). Using this method, we were able to characterize the entire Meishan breeding stock and identify four SLA haplotypes present in the herd. The combination of SLA typing by cloning and DNA sequencing with PCR-SSP is therefore a valuable tool for the characterization of SLA alleles and haplotypes in resource herds of pigs. Springer-Verlag, 2006 Major histocompatibility complex nationallicence Meishan nationallicence Pig nationallicence Sequence-specific primer nationallicence Swine leukocyte antigen nationallicence Ho Chak-Sum Transplant Immunology Laboratory, Department of Pathology, Baylor University Medical Center, 3500 Gaston Avenue, 75246, Dallas, TX, USA aut Rochelle Erin Department of Animal Sciences, 382 Edward R. Madigan Laboratory, University of Illinois, 1201 W. Gregory Drive, 61801, Urbana, IL, USA aut Martens Gregory Transplant Immunology Laboratory, Department of Pathology, Baylor University Medical Center, 3500 Gaston Avenue, 75246, Dallas, TX, USA aut Schook Lawrence Department of Animal Sciences, 382 Edward R. Madigan Laboratory, University of Illinois, 1201 W. Gregory Drive, 61801, Urbana, IL, USA aut Smith Douglas Transplant Immunology Laboratory, Department of Pathology, Baylor University Medical Center, 3500 Gaston Avenue, 75246, Dallas, TX, USA aut Immunogenetics Springer-Verlag 58/11(2006-11-01), 873-882 0093-7711 58:11<873 2006 58 251 https://doi.org/10.1007/s00251-006-0145-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0145-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ho Chak-Sum Transplant Immunology Laboratory, Department of Pathology, Baylor University Medical Center, 3500 Gaston Avenue, 75246, Dallas, TX, USA aut NATIONALLICENCE 700 1- Rochelle Erin Department of Animal Sciences, 382 Edward R. Madigan Laboratory, University of Illinois, 1201 W. Gregory Drive, 61801, Urbana, IL, USA aut NATIONALLICENCE 700 1- Martens Gregory Transplant Immunology Laboratory, Department of Pathology, Baylor University Medical Center, 3500 Gaston Avenue, 75246, Dallas, TX, USA aut NATIONALLICENCE 700 1- Schook Lawrence Department of Animal Sciences, 382 Edward R. Madigan Laboratory, University of Illinois, 1201 W. Gregory Drive, 61801, Urbana, IL, USA aut NATIONALLICENCE 700 1- Smith Douglas Transplant Immunology Laboratory, Department of Pathology, Baylor University Medical Center, 3500 Gaston Avenue, 75246, Dallas, TX, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/11(2006-11-01), 873-882 0093-7711 58:11<873 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer