caa a22 4500 467907714 CHVBK 20180406152839.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s00251-006-0154-x doi (NATIONALLICENCE)springer-10.1007/s00251-006-0154-x Strain-dependent expression of four structurally related rat Ly49 receptors; correlation with NK gene complex haplotype and NK alloreactivity [Elektronische Daten] [Lise Kveberg, Ke-Zheng Dai, Erik Dissen, James Ryan, Bent Rolstad, John Vaage, Christian Naper] Natural killer (NK) cells from certain rat strains promptly kill MHC allogeneic lymphocytes in vivo, a rejection phenomenon termed allogeneic lymphocyte cytotoxicity (ALC). ALC can be reproduced in vitro, and is preferentially mediated by a subset of NK cells expressing the Ly49 stimulatory receptor 3 (Ly49s3) in PVG strain rats. Functional studies have suggested that Ly49s3 triggers NK cell alloreactivity, but its importance relative to other Ly49 receptors has not been investigated. In this study, we have characterized three rat Ly49 receptors with close sequence similarity to Ly49s3 in the extracellular region, i.e., Ly49s4, Ly49 inhibitory receptor 3 (Ly49i3), and Ly49i4. Similar to Ly49s3, Ly49s4 mediated cellular activation while Ly49i4 inhibited NK cytolytic function. Ly49s4, -i3, and -i4 all reacted with a previously described anti-Ly49s3 monoclonal antibody (mAb) (DAR13), but not a novel mAb (STOK6), which was shown to be specific for Ly49s3. Expression of these Ly49 receptors varied markedly between inbred strains, in patterns related to their NK gene complex (NKC) haplotype, and ability to mediate ALC. Three major groups of NKC haplotypes could be discerned by restriction fragment length polymorphism analysis. Ly49s3 was present in strains from one of the groups, which corresponded with the "high” ALC responders. Ly49s3 surface expression was also markedly reduced in the presence of its putative MHC class Ib ligand(s) in MHC congenic strains. These data support the notion that Ly49s3 functions as a triggering MHC receptor both in vitro and in vivo. MHC ligands for the other three Ly49 receptors remain to be determined. Springer-Verlag, 2006 Natural Killer cells nationallicence MHC nationallicence Ly49 receptors nationallicence Alloreactivity nationallicence Rodent nationallicence Kveberg Lise Institute of Immunology (IMMI), Rikshospitalet-Radiumhospitalet Medical Centre, University of Oslo, 0027, Oslo, Norway aut Dai Ke-Zheng Institute of Immunology (IMMI), Rikshospitalet-Radiumhospitalet Medical Centre, University of Oslo, 0027, Oslo, Norway aut Dissen Erik Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, 0317, Oslo, Norway aut Ryan James Veterans Affairs Medical Center, University of California-San Francisco, 94121, San Francisco, CA, USA aut Rolstad Bent Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, 0317, Oslo, Norway aut Vaage John Institute of Immunology (IMMI), Rikshospitalet-Radiumhospitalet Medical Centre, University of Oslo, 0027, Oslo, Norway aut Naper Christian Institute of Immunology (IMMI), Rikshospitalet-Radiumhospitalet Medical Centre, University of Oslo, 0027, Oslo, Norway aut Immunogenetics Springer-Verlag 58/11(2006-11-01), 905-916 0093-7711 58:11<905 2006 58 251 https://doi.org/10.1007/s00251-006-0154-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0154-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kveberg Lise Institute of Immunology (IMMI), Rikshospitalet-Radiumhospitalet Medical Centre, University of Oslo, 0027, Oslo, Norway aut NATIONALLICENCE 700 1- Dai Ke-Zheng Institute of Immunology (IMMI), Rikshospitalet-Radiumhospitalet Medical Centre, University of Oslo, 0027, Oslo, Norway aut NATIONALLICENCE 700 1- Dissen Erik Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, 0317, Oslo, Norway aut NATIONALLICENCE 700 1- Ryan James Veterans Affairs Medical Center, University of California-San Francisco, 94121, San Francisco, CA, USA aut NATIONALLICENCE 700 1- Rolstad Bent Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, 0317, Oslo, Norway aut NATIONALLICENCE 700 1- Vaage John Institute of Immunology (IMMI), Rikshospitalet-Radiumhospitalet Medical Centre, University of Oslo, 0027, Oslo, Norway aut NATIONALLICENCE 700 1- Naper Christian Institute of Immunology (IMMI), Rikshospitalet-Radiumhospitalet Medical Centre, University of Oslo, 0027, Oslo, Norway aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/11(2006-11-01), 905-916 0093-7711 58:11<905 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer