caa a22 4500 467907803 CHVBK 20180406152840.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s00251-005-0060-7 doi (NATIONALLICENCE)springer-10.1007/s00251-005-0060-7 Molecular characterization of KIR3DL3 [Elektronische Daten] [Anita Trundley, Susan Hiby, Chiwen Chang, Andrew Sharkey, Simeon Santourlidis, Markus Uhrberg, John Trowsdale, Ashley Moffett] Killer-cell immunoglobulin-like receptors (KIRs) are a structurally and functionally diverse family of molecules expressed by natural killer (NK) cells and T-cell subsets. The most centromeric gene in the human KIR cluster is KIR3DL3, a framework gene that is present in all haplotypes. KIR3DL3 has only one immunoreceptor tyrosine-based inhibitory motif and lacks the exon encoding the stem between the Immunoglobulin domains and the transmembrane region. We have investigated expression of KIR3DL3 in blood and decidual NK cells by reverse transcriptase polymerase chain reaction (RT-PCR) and protein analysis using a KIR3DL3-specific monoclonal antibody, CH21. KIR3DL3 mRNA was only detected in the CD56bright subset in cells from peripheral blood and in CD56bright decidual NK cells. The CD56bright NK92 cell line was also positive. Quantitative RT-PCR indicated a trend for higher expression of KIR3DL3 in female peripheral blood mononuclear cells compared to that in male. Using a bisulphite conversion method, we found that the promoter of KIR3DL3 was strongly methylated. Surface protein expression was detectable after demethylation. Like other KIRs, KIR3DL3 is highly polymorphic, and we detected 14 variants in 25 unrelated individuals. Nucleotide substitutions were scattered throughout the sequence, with a cluster of alleles at the start of the transmembrane region at the site where the remnant of the linking stem present in other KIR is found. We conclude that the KIR3DL3 gene is not a pseudogene but encodes a protein that is not expressed in healthy individuals. Protein expression might be induced under certain developmental or pathological situations. Springer-Verlag, 2006 Human nationallicence Natural killer cells nationallicence Cell surface molecules nationallicence Reproductive immunology nationallicence Trundley Anita Department of Pathology, Tennis Court Road, CB2 1QP, Cambridge, UK aut Hiby Susan Department of Pathology, Tennis Court Road, CB2 1QP, Cambridge, UK aut Chang Chiwen Department of Pathology, Tennis Court Road, CB2 1QP, Cambridge, UK aut Sharkey Andrew Department of Pathology, Tennis Court Road, CB2 1QP, Cambridge, UK aut Santourlidis Simeon Institute for Transplantation Diagnostics and Cell Therapeutics, Moorenstr. 5, Building 14.80, 40225, Dusseldorf, Germany aut Uhrberg Markus Institute for Transplantation Diagnostics and Cell Therapeutics, Moorenstr. 5, Building 14.80, 40225, Dusseldorf, Germany aut Trowsdale John Department of Pathology, Tennis Court Road, CB2 1QP, Cambridge, UK aut Moffett Ashley Department of Pathology, Tennis Court Road, CB2 1QP, Cambridge, UK aut Immunogenetics Springer-Verlag 57/12(2006-01-01), 904-916 0093-7711 57:12<904 2006 57 251 https://doi.org/10.1007/s00251-005-0060-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-005-0060-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Trundley Anita Department of Pathology, Tennis Court Road, CB2 1QP, Cambridge, UK aut NATIONALLICENCE 700 1- Hiby Susan Department of Pathology, Tennis Court Road, CB2 1QP, Cambridge, UK aut NATIONALLICENCE 700 1- Chang Chiwen Department of Pathology, Tennis Court Road, CB2 1QP, Cambridge, UK aut NATIONALLICENCE 700 1- Sharkey Andrew Department of Pathology, Tennis Court Road, CB2 1QP, Cambridge, UK aut NATIONALLICENCE 700 1- Santourlidis Simeon Institute for Transplantation Diagnostics and Cell Therapeutics, Moorenstr. 5, Building 14.80, 40225, Dusseldorf, Germany aut NATIONALLICENCE 700 1- Uhrberg Markus Institute for Transplantation Diagnostics and Cell Therapeutics, Moorenstr. 5, Building 14.80, 40225, Dusseldorf, Germany aut NATIONALLICENCE 700 1- Trowsdale John Department of Pathology, Tennis Court Road, CB2 1QP, Cambridge, UK aut NATIONALLICENCE 700 1- Moffett Ashley Department of Pathology, Tennis Court Road, CB2 1QP, Cambridge, UK aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 57/12(2006-01-01), 904-916 0093-7711 57:12<904 2006 57 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer