caa a22 4500 467911851 CHVBK 20180406152851.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s11157-006-0009-9 doi (NATIONALLICENCE)springer-10.1007/s11157-006-0009-9 Thermostable Proteins as Probe for the Design of Advanced Fluorescence Biosensors [Elektronische Daten] [Marcella de Champdoré, Maria Staiano, Vincenzo Aurilia, Olesia Stepanenko, Antonietta Parracino, Mosè Rossi, Sabato D'Auria] In this review we explore the advantages deriving from the use of either enzymes or sugar binding proteins isolated from thermophilic organisms to develop stable fluorescence biosensors. We report on a novel approach to address the consumption of the analyte by enzyme-based biosensors, namely the utilization of apo-enzymes as non-active forms of proteins which are still able to bind the ligand but cannot transform it into product. We also report recent studies in which the fluorescence labeling of a naturally thermostable binding protein allows a quantitative determination of glucose. Springer Science+Business Media B.V., 2006 Glucose nationallicence Proteins nationallicence Thermophilic Organisms nationallicence Fluorescence nationallicence Biosensors nationallicence IAANS : 2-(4′-(iodoacetamido)anilino) naphthalene- 6-sulfonic acid (IAANS) nationallicence ANS : 1-(anilino)-naphtalene-8-sulfonate nationallicence FRET : fluorescence resonance energy transfer nationallicence LED : light emitting diode nationallicence GD : glucose dehydrogenase nationallicence BSGK : glucokinase from Bacillus stearothermophilus nationallicence Ph-SBP : sugar-binding protein from Pyrococcus horikoshii nationallicence de Champdoré Marcella Institute of Protein Biochemistry, Italian National Research Council, Via Pietro Castellino 111, 80131, Naples, Italy aut Staiano Maria Institute of Protein Biochemistry, Italian National Research Council, Via Pietro Castellino 111, 80131, Naples, Italy aut Aurilia Vincenzo Institute of Protein Biochemistry, Italian National Research Council, Via Pietro Castellino 111, 80131, Naples, Italy aut Stepanenko Olesia Institute of Protein Biochemistry, Italian National Research Council, Via Pietro Castellino 111, 80131, Naples, Italy aut Parracino Antonietta Institute of Protein Biochemistry, Italian National Research Council, Via Pietro Castellino 111, 80131, Naples, Italy aut Rossi Mosè Institute of Protein Biochemistry, Italian National Research Council, Via Pietro Castellino 111, 80131, Naples, Italy aut D'Auria Sabato Institute of Protein Biochemistry, Italian National Research Council, Via Pietro Castellino 111, 80131, Naples, Italy aut Reviews in Environmental Science and Bio/Technology Kluwer Academic Publishers 5/2-3(2006-08-01), 233-242 1569-1705 5:2-3<233 2006 5 11157 https://doi.org/10.1007/s11157-006-0009-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11157-006-0009-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- de Champdoré Marcella Institute of Protein Biochemistry, Italian National Research Council, Via Pietro Castellino 111, 80131, Naples, Italy aut NATIONALLICENCE 700 1- Staiano Maria Institute of Protein Biochemistry, Italian National Research Council, Via Pietro Castellino 111, 80131, Naples, Italy aut NATIONALLICENCE 700 1- Aurilia Vincenzo Institute of Protein Biochemistry, Italian National Research Council, Via Pietro Castellino 111, 80131, Naples, Italy aut NATIONALLICENCE 700 1- Stepanenko Olesia Institute of Protein Biochemistry, Italian National Research Council, Via Pietro Castellino 111, 80131, Naples, Italy aut NATIONALLICENCE 700 1- Parracino Antonietta Institute of Protein Biochemistry, Italian National Research Council, Via Pietro Castellino 111, 80131, Naples, Italy aut NATIONALLICENCE 700 1- Rossi Mosè Institute of Protein Biochemistry, Italian National Research Council, Via Pietro Castellino 111, 80131, Naples, Italy aut NATIONALLICENCE 700 1- D'Auria Sabato Institute of Protein Biochemistry, Italian National Research Council, Via Pietro Castellino 111, 80131, Naples, Italy aut NATIONALLICENCE 773 0- Reviews in Environmental Science and Bio/Technology Kluwer Academic Publishers 5/2-3(2006-08-01), 233-242 1569-1705 5:2-3<233 2006 5 11157 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer