caa a22 4500 467928177 CHVBK 20180406152935.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s10266-005-0059-4 doi (NATIONALLICENCE)springer-10.1007/s10266-005-0059-4 Expression of keratin 18 in the periderm cells of the lingual epithelium of fetal rats: visualization by fluorescence immunohistochemistry and differential interference contrast microscopy [Elektronische Daten] [Shin-ichi Iwasaki, Hidekazu Aoyagi, Tomoichiro Asami] We examined the expression of keratin 18 (K18), by immunofluorescence staining, while monitoring morphological changes in the periderm on the lingual epithelium of rats by laser-scanning microscopy of epoxy resin-embedded, semi-ultrathin sections. We also examined differential interference contrast (DIC) images of the same sections to define the histology and morphology of the cells. It is difficult to visualize histological details of the fetal lingual epithelium of the rat on semi-ultrathin sections by light microscopy after immunohistochemical staining, because the histological structures in such sections cannot be distinguished by standard counterstaining. To solve this problem and to visualize keratin 18 (K18), we used a combination of immunofluorescence staining of semi-ultrathin sections and corresponding differential contrast (DIC) images, obtained by laser-scanning microscopy. The Society of The Nippon Dental University, 2006 Periderm nationallicence Lingual epithelium nationallicence Keratin 18 nationallicence Immunohistochemistry nationallicence Laser-scanning microscopy nationallicence Iwasaki Shin-ichi Advanced Research Center, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, 951-8580, Niigata, Japan aut Aoyagi Hidekazu Advanced Research Center, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, 951-8580, Niigata, Japan aut Asami Tomoichiro Gunma PAZ College School of Nursing, Gunma, Japan aut Odontology Springer-Verlag 94/1(2006-09-01), 64-68 1618-1247 94:1<64 2006 94 10266 https://doi.org/10.1007/s10266-005-0059-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10266-005-0059-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Iwasaki Shin-ichi Advanced Research Center, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, 951-8580, Niigata, Japan aut NATIONALLICENCE 700 1- Aoyagi Hidekazu Advanced Research Center, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, 951-8580, Niigata, Japan aut NATIONALLICENCE 700 1- Asami Tomoichiro Gunma PAZ College School of Nursing, Gunma, Japan aut NATIONALLICENCE 773 0- Odontology Springer-Verlag 94/1(2006-09-01), 64-68 1618-1247 94:1<64 2006 94 10266 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer