caa a22 4500 467928193 CHVBK 20180406152935.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s10266-006-0057-1 doi (NATIONALLICENCE)springer-10.1007/s10266-006-0057-1 N-Methyl-D-Aspartate (NMDA) and non-NMDA receptor antagonists suppress the superior sagittal sinus-evoked activity of C1 spinal neurons responding to tooth pulp electrical stimulation in rats [Elektronische Daten] [Yoshinobu Fujimi, Mamoru Takeda, Takeshi Tanimoto, Shigeji Matsumoto] The aim of the present study was to determine whether there is a convergence of inputs from tooth pulp (TP) and the superior sagittal sinus (SSS) on rat C1 spinal neurons, and to examine the effects of iontophoretically applied N-methyl-d-aspartate (NMDA) and non-NMDA receptor antagonists on the SSS-evoked activity of C1 neurons. Extracellular single unit-recordings were made from 20 C1 units responding to TP electrical stimulation with a constant temporal relationship to a digastric electromyogram signal, using a multibarrel electrode in pentobarbital-anesthetized rats. Ninety percent of C1 neurons (18/20) responding to TP stimulation also responded to the SSS stimulation. These neurons were considered to be SSS-afferent inputs from Aδ-fibers (5.8 ± 0.6 m/s; n = 18), based on the calculation of nerve conduction velocity. After the iontophoretic application (30, 50, and 70 nA) of an NMDA receptor blocker (5R-10S)-(+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d] cycloheptene-5,10-imine hydrogen maleate (MK801) or a non-NMDA receptor blocker (6-cyano-7-nitroquinoxaline-2,3-dione) (CNQX), the mean number of spikes responding to the SSS stimulation significantly decreased (30, 50, and 70 nA; P < 0.05). These results suggest that there is a convergence of inputs from SSS and TP afferents on C1 neurons; it is possible that both NMDA and non-NMDA receptors located on C1 neurons may be targets for the treatment of the trigeminal referred pain associated with migraine. The Society of The Nippon Dental University, 2006 C1 spinal neuron nationallicence Tooth pulp nationallicence Superior sagittal sinus nationallicence Referred pain nationallicence Fujimi Yoshinobu Department of Physiology, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, 102-8159, Tokyo, Japan aut Takeda Mamoru Department of Physiology, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, 102-8159, Tokyo, Japan aut Tanimoto Takeshi Department of Physiology, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, 102-8159, Tokyo, Japan aut Matsumoto Shigeji Department of Physiology, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, 102-8159, Tokyo, Japan aut Odontology Springer-Verlag 94/1(2006-09-01), 22-28 1618-1247 94:1<22 2006 94 10266 https://doi.org/10.1007/s10266-006-0057-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10266-006-0057-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fujimi Yoshinobu Department of Physiology, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, 102-8159, Tokyo, Japan aut NATIONALLICENCE 700 1- Takeda Mamoru Department of Physiology, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, 102-8159, Tokyo, Japan aut NATIONALLICENCE 700 1- Tanimoto Takeshi Department of Physiology, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, 102-8159, Tokyo, Japan aut NATIONALLICENCE 700 1- Matsumoto Shigeji Department of Physiology, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, 102-8159, Tokyo, Japan aut NATIONALLICENCE 773 0- Odontology Springer-Verlag 94/1(2006-09-01), 22-28 1618-1247 94:1<22 2006 94 10266 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer