caa a22 4500 467928207 CHVBK 20180406152935.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s10266-006-0065-1 doi (NATIONALLICENCE)springer-10.1007/s10266-006-0065-1 In vitro chromosome aberration tests using human dental pulp cells to detect the carcinogenic potential of chemical agents [Elektronische Daten] [Takeo W. Tsutsui, Tomohiro Inaba, Larry W. Fisher, Pamela Gehron Robey, Takeki Tsutsui] To examine if human dental pulp cells are useful for assessing the carcinogenic potential of chemical agents, we cultured human dental pulp cells from adults and studied the ability of chemical agents known to be carcinogenic to induce chromosome aberrations in these cells. We confirmed that human dental pulp cells in primary or secondary cultures had the capability of accumulating calcium in vitro as detected by Alizarin red staining and generating dentin-like tissue in immunocompromised mice. These phenotypes were maintained even in cells at seven passages. Next, we examined if chromosome aberrations were induced by exposure of human dental pulp cells (designated here as D824 cells) at seven to nine passages to chemical agents with carcinogenic activity. Statistically significant increases in the frequencies of chromosome aberrations were induced in D824 cells treated with a direct-acting carcinogen, mitomycin C, for 3 h. Chromosome aberrations were also induced at statistically significant levels in D824 cells treated with an indirect-acting carcinogen, cyclophosphamide, for 2 h in the presence of exogenous metabolic activation with rat liver postmitochondrial supernatant. Cyclophosphamide failed to induce chromosome aberrations in the absence of exogenous metabolic activation. Although the reliability of chromosome aberration tests using human dental pulp cells remains to be validated by studying the ability of various other chemical agents with or without carcinogenic activity to induce chromosome aberrations, this chromosome aberration test system may be useful for carcinogenic risk assessment in the target cells. The Society of The Nippon Dental University, 2006 Human dental pulp cells nationallicence Chromosome aberrations nationallicence Genetic toxicity nationallicence Mitomycin C nationallicence Cyclophosphamide nationallicence Tsutsui Takeo W. Department of Pharmacology, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, 102-8159, Tokyo, Japan aut Inaba Tomohiro Oral and Maxillofacial Surgery, The Nippon Dental University Hospital at Tokyo, Tokyo, Japan aut Fisher Larry W. Department of Health and Human Services, Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA aut Robey Pamela Gehron Department of Health and Human Services, Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA aut Tsutsui Takeki Department of Pharmacology, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, 102-8159, Tokyo, Japan aut Odontology Springer-Verlag 94/1(2006-09-01), 44-50 1618-1247 94:1<44 2006 94 10266 https://doi.org/10.1007/s10266-006-0065-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10266-006-0065-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tsutsui Takeo W. Department of Pharmacology, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, 102-8159, Tokyo, Japan aut NATIONALLICENCE 700 1- Inaba Tomohiro Oral and Maxillofacial Surgery, The Nippon Dental University Hospital at Tokyo, Tokyo, Japan aut NATIONALLICENCE 700 1- Fisher Larry W. Department of Health and Human Services, Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Robey Pamela Gehron Department of Health and Human Services, Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Tsutsui Takeki Department of Pharmacology, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, 102-8159, Tokyo, Japan aut NATIONALLICENCE 773 0- Odontology Springer-Verlag 94/1(2006-09-01), 44-50 1618-1247 94:1<44 2006 94 10266 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 46269769X BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer