caa a22 4500 467928231 CHVBK 20180406152935.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s10266-006-0063-3 doi (NATIONALLICENCE)springer-10.1007/s10266-006-0063-3 ten Cate Jacob M. Department of Cariology Endodontolgy Pedodontology, Academic Centre for Dentistry (ACTA), Louwesweg 1, 1066 EA, Amsterdam, The Netherlands aut Biofilms, a new approach to the microbiology of dental plaque [Elektronische Daten] [Jacob M. ten Cate] Dental plaque has the properties of a biofilm, similar to other biofilms found in the body and the environment. Modern molecular biological techniques have identified about 1000 different bacterial species in the dental biofilm, twice as many as can be cultured. Oral biofilms are very heterogeneous in structure. Dense mushroom-like structures originate from the enamel surface, interspersed with bacteria-free channels used as diffusion pathways. The channels are probably filled with an extracellular polysaccharide (EPS) matrix produced by the bacteria. Bacteria in biofilms communicate through signaling molecules, and use this "quorum-sensing” system to optimize their virulence factors and survival. Bacteria in a biofilm have a physiology different from that of planktonic cells. They generally live under nutrient limitation and often in a dormant state. Such "sleepy” bacteria respond differently to antibiotics and antimicrobials, because these agents were generally selected in experiments with metabolically active bacteria. This is one of the explanations as to why antibiotics and antimicrobials are not as successful in the clinic as could be expected from laboratory studies. In addition, it has been found that many therapeutic agents bind to the biofilm EPS matrix before they even reach the bacteria, and are thereby inactivated. Taken together, these fundings highlight why the study of bacteria in the oral cavity is now taken on by studying the biofilms rather than individual species. The Society of The Nippon Dental University, 2006 Biofilm nationallicence Structure nationallicence Quorum-sensing nationallicence Antimicrobials nationallicence Odontology Springer-Verlag 94/1(2006-09-01), 1-9 1618-1247 94:1<1 2006 94 10266 https://doi.org/10.1007/s10266-006-0063-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10266-006-0063-3 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- ten Cate Jacob M. Department of Cariology Endodontolgy Pedodontology, Academic Centre for Dentistry (ACTA), Louwesweg 1, 1066 EA, Amsterdam, The Netherlands aut NATIONALLICENCE 773 0- Odontology Springer-Verlag 94/1(2006-09-01), 1-9 1618-1247 94:1<1 2006 94 10266 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer