caa a22 4500 467928738 CHVBK 20180406152937.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s10658-006-9036-1 doi (NATIONALLICENCE)springer-10.1007/s10658-006-9036-1 Differentiation of Clavibacter michiganensis subsp. michiganensis from seed-borne saprophytes using ELISA, Biolog and 16S rDNA sequencing [Elektronische Daten] [Wendy Kaneshiro, Carla Mizumoto, Anne Alvarez] Specificity of a monoclonal antibody (MAb), Cmm1, to geographically diverse strains of the seed-borne tomato pathogen, Clavibacter michiganensis subsp. michiganensis (Cmm), was assessed and the MAb was tested for its usefulness as a tool to separate the pathogen from saprophytes in naturally infested tomato seed. Of the 236 international Cmm strains tested, 99% reacted with MAb Cmm1. MAb Cmm1 was also strongly reactive with an additional 32 strains isolated from seed that were later identified as Cmm by the Biolog MicroLog™ microbial identification system (Biolog, Inc., Hayward, CA) and 16S rDNA sequence analysis. It correctly differentiated these strains from 12 MAb Cmm1-negative seed strains that possessed similar colony morphology but were later identified as other Gram-positive genera and species. The specificity of MAb Cmm1 to the pathogen and the near universality of the MAb Cmm1-reactive antigen among diverse Cmm strains make this antibody a useful detection and identification tool. The finding that a large proportion of the Cmm strains associated with naturally infested tomato seed were putatively hypovirulent or non-virulent indicates that such populations cannot be ignored and points to a need for studies to determine their significance in host-pathogen interactions. Springer Science+Business Media, Inc., 2006 bacterial canker nationallicence detection nationallicence hypovirulent nationallicence MAb Cmm1 nationallicence non-virulent nationallicence seed testing nationallicence Kaneshiro Wendy Department of Plant and Environmental Protection Sciences, University of Hawaii, 3190 Maile Way, St. John Plant Science Building 307, 96822, Honolulu, HI, USA aut Mizumoto Carla Department of Plant and Environmental Protection Sciences, University of Hawaii, 3190 Maile Way, St. John Plant Science Building 307, 96822, Honolulu, HI, USA aut Alvarez Anne Department of Plant and Environmental Protection Sciences, University of Hawaii, 3190 Maile Way, St. John Plant Science Building 307, 96822, Honolulu, HI, USA aut European Journal of Plant Pathology Kluwer Academic Publishers 116/1(2006-09-01), 45-56 0929-1873 116:1<45 2006 116 10658 https://doi.org/10.1007/s10658-006-9036-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-006-9036-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kaneshiro Wendy Department of Plant and Environmental Protection Sciences, University of Hawaii, 3190 Maile Way, St. John Plant Science Building 307, 96822, Honolulu, HI, USA aut NATIONALLICENCE 700 1- Mizumoto Carla Department of Plant and Environmental Protection Sciences, University of Hawaii, 3190 Maile Way, St. John Plant Science Building 307, 96822, Honolulu, HI, USA aut NATIONALLICENCE 700 1- Alvarez Anne Department of Plant and Environmental Protection Sciences, University of Hawaii, 3190 Maile Way, St. John Plant Science Building 307, 96822, Honolulu, HI, USA aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 116/1(2006-09-01), 45-56 0929-1873 116:1<45 2006 116 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer