caa a22 4500 467928789 CHVBK 20180406152937.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s10658-006-9035-2 doi (NATIONALLICENCE)springer-10.1007/s10658-006-9035-2 Development of an effective screening method for partial resistance to Alternaria brassicicola (dark leaf spot) in Brassica rapa [Elektronische Daten] [M. Doullah, M. Meah, K. Okazaki] In order to develop a method to measure resistance to Alternaria brassicicola (cause of dark leaf spot disease) in Brassica rapa, the effects of inoculum concentration, leaf stage, leaf age and incubation temperature of inoculation on infection were studied under controlled conditions using several B. rapa genotypes. Three inoculation methods (cotyledon, detached leaf and seedling inoculation) were evaluated for this purpose. The detached leaf inoculation test was the most suitable for screening B. rapa genotypes because clear symptoms were observed on the leaves in less than 24h, and there was a significant positive correlation between the results from the detached leaf inoculation test and the seedling inoculation test, an established method considered to yield reliable results. In addition, it was very easy to screen plants for resistance on a large scale and to maintain standard physical conditions using detached leaves. For successful infection, inoculum concentration should be adjusted to 5 × 104 conidia ml−1, and incubation temperature should be between 20°C and 25°C. The 3rd/4th true leaves from 30day-old plants were optimal for inoculation. In a screening test using 52 cultivars of B. rapa, the detached leaf test effectively discriminated between various levels of partial resistance among cultivars. As a result, we identified two cultivars, viz Saori and Edononatsu, as highly resistant and five cultivars, viz Tokinashi Taisai, Yajima Kabu, Purara, Norin-F1-Bekana and Tateiwa Kabu, as having borderline resistance. Springer, 2006 inoculum concentration nationallicence incubation temperature nationallicence inoculation technique nationallicence leaf position nationallicence leaf age nationallicence rapeseed nationallicence Doullah M. Faculty of Agriculture, Niigata University, 2-8050, Ikarashi, 950-2181, Niigata, Japan aut Meah M. Department of Plant Pathology, Bangladesh Agricultural University, 2202, Mymensingh, Bangladesh aut Okazaki K. Faculty of Agriculture, Niigata University, 2-8050, Ikarashi, 950-2181, Niigata, Japan aut European Journal of Plant Pathology Kluwer Academic Publishers 116/1(2006-09-01), 33-43 0929-1873 116:1<33 2006 116 10658 https://doi.org/10.1007/s10658-006-9035-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-006-9035-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Doullah M. Faculty of Agriculture, Niigata University, 2-8050, Ikarashi, 950-2181, Niigata, Japan aut NATIONALLICENCE 700 1- Meah M. Department of Plant Pathology, Bangladesh Agricultural University, 2202, Mymensingh, Bangladesh aut NATIONALLICENCE 700 1- Okazaki K. Faculty of Agriculture, Niigata University, 2-8050, Ikarashi, 950-2181, Niigata, Japan aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 116/1(2006-09-01), 33-43 0929-1873 116:1<33 2006 116 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer