caa a22 4500 467928797 CHVBK 20180406152937.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s10658-006-9022-7 doi (NATIONALLICENCE)springer-10.1007/s10658-006-9022-7 Cunnington James Department of Primary Industries, Knoxfield Centre, Ferntree Gully Delivery Centre, Private Bag 15, 3156, Victoria, Australia aut Novel primers developed from mitochondrial intergenic spacers are useful for multi-locus sequence typing of Fusarium oxysporum strains [Elektronische Daten] [James Cunnington] The Fusarium oxysporum species complex causes serious vascular diseases of a wide range of crops. In an effort to locate new highly variable regions for multi-locus sequence typing (MLST) of Fusarium oxysporum populations, mitochondrial intergenic regions were targeted. Five regions were amplified and sequenced from five randomly selected isolates. For these isolates, all regions contained at least as many variable sites as the mitochondrial ribosomal RNA small subunit region commonly used in MLST. Most regions contained short indels; however, 80-120 base indels were present in the nad5-arg2 intergenic region. Only the nad5-arg2 region was found to be more variable than the single copy nuclear translation elongation factor (1α) gene introns. The nad5-arg2 region is likely to be a useful inclusion in MLST of Fusarium oxysporum populations. Springer Science+Business Media, Inc., 2006 Fusarium wilt nationallicence sequence diversity nationallicence European Journal of Plant Pathology Kluwer Academic Publishers 116/1(2006-09-01), 77-80 0929-1873 116:1<77 2006 116 10658 https://doi.org/10.1007/s10658-006-9022-7 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-006-9022-7 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Cunnington James Department of Primary Industries, Knoxfield Centre, Ferntree Gully Delivery Centre, Private Bag 15, 3156, Victoria, Australia aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 116/1(2006-09-01), 77-80 0929-1873 116:1<77 2006 116 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer