caa a22 4500 467928800 CHVBK 20180406152937.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s10658-006-9038-z doi (NATIONALLICENCE)springer-10.1007/s10658-006-9038-z The Use of ARMS PCR in Detection and Identification of Xanthomonads Associated with Pistachio Dieback in Australia [Elektronische Daten] [A. Marefat, K. Ophel-Keller, E. Scott, M. Sedgley] Pistachio dieback occurs in the main pistachio growing areas of Australia. Xanthomonas strains belonging to the translucens group have been identified as the causal agent of the disease and two distinct groups, A and B, have been recognised within the pathogen population. In this study, specific primers for amplification of DNA of the pathogen were developed by sequencing the Internal Transcribed Spacer (ITS) region of rDNA from strains representing groups A and B, as well as from X. translucens isolated from wheat in Australia and one Xanthomonas translucens strain from orchard floor grasses. Primers were designed for amplification of DNA sequences specific to each group and a multiplex PCR test was developed that identified and differentiated strains of each group in a single PCR assay. To determine the specificity of the primers, PCR was carried out with DNA from 65 strains of the pistachio pathogen, 31 type and reference strains of Xanthomonas, and from 191 phytobacteria commonly found in and around pistachio orchards. In the multiplex PCR, a 331bp fragment was amplified from all strains belonging to group A and a 120bp fragment from all strains in group B. No PCR products were obtained from the other bacteria tested except for the type strain of X. translucens pv. cerealis, which has not been found in Australia. The assay was used to detect strains from both groups of the pathogen in pistachio plant material. Springer Science+Business Media B.V., 2006 16S-23S rDNA sequencing nationallicence Multiplex-PCR nationallicence Xanthomonas translucens nationallicence Marefat A. School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, PMB 1, 5064, Glen Osmond, SA, Australia aut Ophel-Keller K. South Australian Research and Development Institute, GPO Box 397, 5001, Adelaide, SA, Australia aut Scott E. School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, PMB 1, 5064, Glen Osmond, SA, Australia aut Sedgley M. Faculty of The Sciences, The University of New England, 2351, Armidale, NSW, Australia aut European Journal of Plant Pathology Kluwer Academic Publishers 116/1(2006-09-01), 57-68 0929-1873 116:1<57 2006 116 10658 https://doi.org/10.1007/s10658-006-9038-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10658-006-9038-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Marefat A. School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, PMB 1, 5064, Glen Osmond, SA, Australia aut NATIONALLICENCE 700 1- Ophel-Keller K. South Australian Research and Development Institute, GPO Box 397, 5001, Adelaide, SA, Australia aut NATIONALLICENCE 700 1- Scott E. School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, PMB 1, 5064, Glen Osmond, SA, Australia aut NATIONALLICENCE 700 1- Sedgley M. Faculty of The Sciences, The University of New England, 2351, Armidale, NSW, Australia aut NATIONALLICENCE 773 0- European Journal of Plant Pathology Kluwer Academic Publishers 116/1(2006-09-01), 57-68 0929-1873 116:1<57 2006 116 10658 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer